Project description:Using an integrated systems approach, the expressed proteome of B. diazoefficiens strain 110scp4 was measured under i) normal, oxic growth, and ii) microoxic growth condtions. This included, as a first step, the sequencing and de novo assembly of the genome of this widely used rhizobial model strain, which turned out to harbor several deletions and insertions compared to the B. diazoefficiens USDA 110 NCBI reference genome. With this optimal basis in hand, a shotgun proteomics approach relying on a slightly adapated FASP protocol was carried out, allowing to identify 2900 (oxia) and 2826 (microoxia) proteins, respectively, thereby largely expanding the proteome known to be expressed under microoxic conditions.

2019-05-30 | PXD012491 | Pride

Proteomic Characterization of Bradyrhizobium diazoefficiens bacteroids during soybean root nodules senescence

Project description:The form and physiology of Bradyrhizobium diazoefficiens after the decline of symbiotic nitrogen fixation has been characterized. Proteomic analyses showed that the post-symbiotic B. diazoefficiens undergo metabolic remodeling as well defined groups of proteins declined, increased or remained unchanged from 56 to 119 days after planting suggesting a transition to a hemibiotrophic-like lifestyle. Enzymatic analysis showed distinct patterns in both the cytoplasm and the periplasm. Similar to the bacteroid, the post-symbiotic bacteria rely on a non-citric acid cycle supply of succinate and although viable, they did not demonstrate the ability to grow within the senescent nodule.

2019-11-12 | PXD011604 | Pride

Bradyrhizobium diazoefficiens strain:SEMIA 586

Project description:Bradyrhizobium diazoefficiens strain:SEMIA 586 Genome sequencing and assembly

| PRJNA787643 | ENA

Bradyrhizobium diazoefficiens strain:CPAC 390

Project description:Bradyrhizobium diazoefficiens strain:CPAC 390 Genome sequencing and assembly

| PRJNA787647 | ENA