Project description:This is the first metaproteomics-based featuring of the microbial community harbured in the traditional raw milk Caprino Nicastrese cheese

2023-03-11 | PXD032280 | Pride

Fuscospora alessandri breed:Los Ruiles

Project description:Fuscospora alessandri breed:Los Ruiles Raw sequence reads

| PRJNA702808 | ENA

Minas Frescal Cheese

Project description:Minas Frescal Cheese Raw sequence reads

| PRJNA514241 | ENA

Adult Needles timelapse and whorl - Los Reales 2012

Project description:Transcriptional profiling of Pinus pinaster adult needles in a complete year. The needles were isolated by year emergence (whorl) in four groups. 0 (2012), 1 (2011), 2 (2010) and 3 (2009). The samples were harvested at 1245 m of altitude at Los Reales de Sierra Bermeja (Spain) (30S X:303.095 Y:4.039.618) one time per month. Reference design. 6 biological replicates that were pooled two by two resulting in 3 samples for the hybridization. The common reference samples was a pool of RNA samples from January. The reference sample was labeled with Cy5 and the test samples with Cy3.

2015-02-27 | E-GEOD-55868 | biostudies-arrayexpress

Transcriptomic profiles of six strains of Lactococcus lactis in ultrafiltration-cheese model

Project description:The intra sub-species diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in UF-cheese model. Six strains were isolated from various sources, but all are exhibiting a dairy phenotype. Our results showed that, the six strains exhibited small phenotypic differences since similar behaviour in terms of growth was obtained during cheese ripening while only different acidification capability was detected. Even if all strains displayed high genomic similarities, sharing a high core genome of almost two thousands genes, the expression of this core genome directly in the cheese matrix revealed major strain-specific differences. This strains with the same dairy origin. Strains were cultured on skimmed raw milk ultrafiltration (UF) retentate. The UF retentate was pre-incubated overnight at 4 °C, then 45 minutes at 50 °C and homogenized during 1.5 minutes at 24 000 rpm with an ultra-turax (Imlab, France). After addition of rennet (0.3 µl ml-1), 400 g UF retentate was inoculated at 2 106 CFU/g with L. lactis subsp. lactis strains. After incubation for 8 hours at 30 °C, the cheeses were transferred at 12° C until 7 days for ripening simulation. At least three independent cultures of the six strains were performed. Total RNA was extracted from cells grown 24 hours in UF-cheese and radiolabelled cDNA were prepared and hybridized on nylon arrays. 1948 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. 3 independent repetitions were performed.

2012-09-07 | E-GEOD-23987 | biostudies-arrayexpress

Microbiota and mycobiota of Queijo de Azeitao PDO cheese

Project description:Microbiota and mycobiota of Queijo de Azeitao PDO cheese Metagenome

| PRJNA706919 | ENA