Project description:To define the IL-23R-dependent changes in intestinal regulatory T cells (Treg cells) that might contribute to the impact on Treg cell function and selective advantage

2023-02-17 | GSE208621 | GEO

Transcriptional analysis of human IL-23R mediated apoptosis in human cervical cancer cells in vitro

Project description:We found that IL-23R mediated HeLa cell apoptosis by characterizing the functional domains responsible for this event. For exploring apoptotic pathways activated by IL-23R, the altered transcriptomic profiles in responding to IL-23R over-expression was also revealed by RNAseq analysis.

2023-04-06 | GSE229036 | GEO

Identification of a colitogenic memory CD4+ T cell population that mediates gastrointestinal GVHD

Project description:Using an experimental model of graft versus host disease (GVHD) to examine T cell-mediated inflammation within the colon, we identified a unique CD4+ T cell population that constitutively expresses the β2 integrin, CD11c, has a biased central memory phenotype and memory T cell transcriptional profile, possesses innate-like properties by gene expression analysis, and has increased expression of the gut-homing molecules, α4β7 and CCR9. Using a number of complementary GVHD mouse models, we show that adoptive transfer of these cells results in TH1-mediated proinflammatory cytokine production, augmented pathological damage in the colon, and increased mortality due to early accumulation of these cells in the GI tract. The pathogenic effects of this CD4+ T cell population was critically dependent upon co-expression of the IL-23 receptor which was required for maximal inflammatory effects. Colonic inflammation was regulated by IL-10 that was produced by non-Foxp3-expressing CD4+ T cells which attenuated lethality in the absence of functional CD4+ Foxp3+ T cells. Thus, coordinate expression of CD11c and the IL-23R defines a novel IL-10 regulated, colitogenic memory CD4+ T cell subset that is poised to initiate inflammation when there is loss of tolerance and breakdown of mucosal barriers as occurs in GVHD as well as other immune-mediated inflammatory bowel disorders.

2016-08-12 | GSE83552 | GEO

Induction of a colitogenic phenotype in Th1-like cells depends on interleukin-23 receptor signaling [in vitro]

Project description:Single-cell RNA-sequencing of in vitro differentiated T cells cultured with IL-12+IL-21+IL-23 (Th1 cell condition) or IL-1b+IL-6+IL-23 (pathogenic Th17 cell condition)

2021-05-01 | GSE164518 | GEO

Induction of a colitogenic phenotype in Th1-like cells depends on interleukin-23 receptor signaling [in vivo]

Project description:Th1 cells were adoptively transferred into RAG1 KO mice from either wildtype (Il23rwt/eGFP) or KO (Il23reGFP/eGFP) donors. At the peak of disease in recipients of wildtype cells, the animals were euthanized and intestinal T cells were isolated from the chosen recipients of both wildtype and KO cells for 10x massively parallel single-cell RNA-sequencing.

2021-05-01 | GSE164519 | GEO

IL-23R-activated STAT3/STAT4 is essential for Th1/Th17-mediated CNS autoimmunity.

Project description: Not available

| S-EPMC5621925 | biostudies-literature