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Transcriptomic analysis of the hypothalamic paraventricular and the arcuate nucleus of NNAT null mice

ABSTRACT: Transcriptomic analysis of the hypothalamic paraventricular and the arcuate nucleus of NNAT null mice

PROVIDER: PRJNA718562 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of the hypothalamic paraventricular and the arcuate nucleus of NNAT null mice

Project description:Neuronatin (Nnat) has previously been reported to be part of a network of imprinted genes. Disruption of neuronatin results in an unusual phenotype of a bimodal body weight.In order to understand at the molecular level how the Nnat deficiency could contribute to the hypervariable phenotypes seen, we performed RNA sequencing from laser-capture micro dissected paraventricular nucleus (PVN) and arcuate nucleus (ARC) and compared the transcriptome between Nnat+/-p mice and their Nnat+/+ littermates in different feeding conditions. We performed an analysis of three subgroups: Nnat+/+ (all non-obese), Nnat+/-p non-obese, and Nnat+/-p obese mice and combined two approaches to identify differences in hypothalamic gene expression between subgroups

2021-09-03 | GSE171155 | GEO

Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide chromatin accessibility patterns in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed ATAC-seq on a genome-wide scale. Combined with previous RRBS, RRHP and RNA-seq, the connections between DNA (hydroxyl)methylation in retroelements and gene expression were studied, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

2021-04-02 | E-MTAB-10263 | biostudies-arrayexpress

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide DNA methylation and hydroxymethylation patterns as well as the transcription profiles in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed RNA-seq, reduced representation bisulfite sequencing (RRBS) and hydroxymethylation profiling (RRHP) on a genome-wide scale. We identified a large number of differential expressed genes (DEGs) and differential 5(h)mC signals across the whole genome. We discovered novel connections between DNA (hydroxyl)methylated modification and gene expression, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

2020-08-21 | E-MTAB-9421 | biostudies-arrayexpress

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

2020-08-20 | E-MTAB-9420 | biostudies-arrayexpress

Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

| PRJEB43863 | ENA

Transcription Factors Dlx1 and Otp Segregate the Identity of Orexigenic/Anti-thermogenic- and Growth Promoting-Neurons in the Arcuate Nucleus

Project description:The hypothalamic arcuate nucleus contains multiple types of neurons controlling critical physiological processes. However, the gene regulatory network directing their development remains rudimentary. Here we report that two transcription factors Otp and Dlx1 segregate the identity of orexigenic/anti-thermogenic NPY/AgRP- and growth-promoting GHRH-neurons in the developing arcuate nucleus. Otp-null and Dlx1-null mice lose NPY/AgRP and GHRH expression, respectively. Dlx1-null mice also show enhanced expression of Otp/NPY/AgRP, which is normalized in Dlx1;Otp-double mutant mice. Correspondingly, Dlx1-null mice exhibit decreased growth, lower body temperature and increased feeding. Furthermore, our genome-wide studies identify Otp as a negative target gene of Dlx1. Therefore, Otp is critical for NPY/AgRP-neuronal development, while Dlx1 promotes GHRH-neuronal development and antagonizes NPY/AgRP-neuronal development. These results identify a mechanism for segregating the identity of two functionally related neurons, and the Dlx1-Otp axis likely contributes to coordinating energy balance and growth by maintaining a proper ratio of NPY/AgRP- to GHRH-neurons.

2018-05-31 | GSE104372 | GEO

ATAC-seq of iPSC-derived hypothalamic arcuate-like neuron cells

Project description:We performed ATAC-seq on iPSC-derived hypothalamic arcuate-like neuron cells to identify putative regulatory elements. All samples were derived from the same individual and from the same differentiation/cell line but ATAC-seq was performed in 3 separate experiments (3 technical replicates).

2021-04-01 | E-MTAB-10257 | biostudies-arrayexpress

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

Project description:Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

| PRJEB39790 | ENA

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

Project description:Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

| PRJEB39815 | ENA