Project description:With HiRIEF LC-MS/MS shotgun proteomics, we analysed 6 patient-derived glioblastoma stem cells (BT stem cells) and compared them to an astrocyte line (CliniSciences, Guidonia Montecelio, Italy) and a more differentiated glioblastoma cell line (T98G). Each of the 8 cell line sample was run in triplicates in a total of three TMT10 sets, assigning each replicate in a separate TMT10 set, using 2 internal standards per set. The three TMT10 sets were ran in two experiments, first on immobilized pH gradient (IPG) 3-10 isoelectric focusing (IEF) strips, and then on IPG 3.7-4.9 IEF strips.

2022-12-23 | PXD027335 | Pride

Quantitative phosphoproteome analysis of glioblastoma stem cells

Project description:Glioblastoma is one of the most malignant brain tumors with poor prognosis and their development and progression are known to be driven by glioblastoma stem cells. Although glioblastoma stem cells lose their cancer stemness properties during cultivation in serum-containing medium, little is known about the molecular mechanisms regulating signaling alteration in relation to reduction of stemness. In order to elucidate the global phosphorylation-related signaling events, we performed a SILAC-based quantitative phosphoproteome analysis of serum-induced dynamics in glioblastoma stem cells established from the tumor tissues of the patient. Among a total of 2,876 phosphorylation sites on 1,584 proteins identified in our analysis, 732 phosphorylation sites on 419 proteins were regulated through the alteration of stem cell characteristics.

2016-01-13 | PXD002614 | Pride

Epigenetic characterization of glioblastoma stem cells

Project description:Epigenetic characterization of glioblastoma stem cells

| PRJNA430732 | ENA

YTHDF2 iCLIP in glioblastoma stem cells

Project description:YTHDF2 iCLIP in glioblastoma stem cells

| PRJNA663772 | ENA

Expression profiling of glioblastoma neural stem cells and reprogrammed derivatives

Project description:Glioblastoma-derived neural stem (GNS) cells were reprogrammed to induced pluripotent stem (iPS) cells by transgenic expression of OCT4 and KLF4. Gene expression profiling was performed in comparison to normal neural stem (NS) cells reprogrammed in parallel, as well as standard ES cells as an independent reference.

2013-03-15 | E-MTAB-1273 | biostudies-arrayexpress

RNAseq Analysis in glioblastoma cells treated with Mepazine

Project description:RNAseq Analysis in glioblastoma cells treated with Mepazine

| PRJNA578094 | ENA