Proteomics

Dataset Information

75

Quantitative phosphoproteome analysis of glioblastoma stem cells


ABSTRACT: Glioblastoma is one of the most malignant brain tumors with poor prognosis and their development and progression are known to be driven by glioblastoma stem cells. Although glioblastoma stem cells lose their cancer stemness properties during cultivation in serum-containing medium, little is known about the molecular mechanisms regulating signaling alteration in relation to reduction of stemness. In order to elucidate the global phosphorylation-related signaling events, we performed a SILAC-based quantitative phosphoproteome analysis of serum-induced dynamics in glioblastoma stem cells established from the tumor tissues of the patient. Among a total of 2,876 phosphorylation sites on 1,584 proteins identified in our analysis, 732 phosphorylation sites on 419 proteins were regulated through the alteration of stem cell characteristics.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo sapiens  

TISSUE(S): Brain

DISEASE(S): Brain Cancer

SUBMITTER: Yuta Narushima  

LAB HEAD: Masaaki Oyama

PROVIDER: PXD002614 | Pride | 2016-01-13

REPOSITORIES: Pride

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Publications

Integrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals Transforming Growth Factor-beta Receptor type-2 (TGFBR2) as a Novel Regulator of Glioblastoma Stem Cell Properties.

Narushima Yuta Y   Kozuka-Hata Hiroko H   Koyama-Nasu Ryo R   Tsumoto Kouhei K   Inoue Jun-ichiro J   Akiyama Tetsu T   Oyama Masaaki M  

Molecular & cellular proteomics : MCP 20151215 3


Glioblastoma is one of the most malignant brain tumors with poor prognosis and their development and progression are known to be driven by glioblastoma stem cells. Although glioblastoma stem cells lose their cancer stem cell properties during cultivation in serum-containing medium, little is known about the molecular mechanisms regulating signaling alteration in relation to reduction of stem cell-like characteristics. To elucidate the global phosphorylation-related signaling events, we performed  ...[more]

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