Project description:Although the biodegradation of biodegradable plastics in soil and compost is well-studied, there is little knowledge on the metabolic mechanisms of synthetic polymers degradation by marine microorganisms. Here, we present a multiomics study to elucidate the biodegradation mechanism of a commercial aromatic-aliphatic copolyester film by a marine microbial enrichment culture. The plastic film and each monomer can be used as sole carbon source. Our analysis showed that the consortium synergistically degrades the polymer, different degradation steps being performed by different members of the community. Analysis of gene expression and translation profiles revealed that the relevant degradation processes in the marine consortium are closely related to poly(ethylene terephthalate) biodegradation from terrestrial microbes. Although there are multiple genes and organisms with the potential to perform a degradation step, only a few of these are active during biodegradation. Our results elucidate the potential of marine microorganisms to mineralize biodegradable plastic polymers and describe the mechanisms of labor division within the community to get maximum energetic yield from a complex synthetic substrate.
2020-09-25 | PXD018391 | Pride
Project description:Biodegradation of E2 under aerobic and anaerobic conditions
| PRJNA558281 | ENA
Project description:Biodegradation of E2 under aerobic and anaerobic conditions - Archaea
Project description:The goal of this study is to obtain a genomic view of the Fur regulatory network under both iron replete and iron deficient conditions in Bacillus subtilis using ChIP-seq. Besides the known Fur target sites, 70 putative DNA binding sites were identified, and the vast majority had higher occupancy under iron sufficient conditions. In addition,we discovered a role for catechol degradation in bacillibactin metabolism, and provided evidence that catechol 2,3-dioxygenase can detoxify endogenously produced catechol substrates in addition to its more widely studied role in biodegradation of environmental aromatic compounds and pollutants.
Project description:To understand how mechanical cues regulate cell metabolism, we compared early changes in gene expression in Ras-transformed MCF10AtK1 mammary epithelial cells grown in high cytoskeletal tension conditions (plastics) vs. cells grown in low cytoskeletal tension conditions (inhibition of the non-muscle myosin II regulatory kinases ROCK and MLCK with standard small-molecule inhibitors Y27632 and ML7, respectively). Keywords: Expression profiling by array
2019-02-05 | GSE107275 | GEO
Project description:Biodegradation of plastics from waste electrical and electronic equipment by Greater Wax Moth Larvae (Galleria mellonella)
Project description:Studies of expression of mechanims of defense of the Acinetobacter sp.5-2Ac.02 from airborne hospital environment under stress conditions, such as SOS response (ROS response, heavy metals resistant mechanisms, peptides), as well as Quorum network (acetoin cluster and aromatics biodegradation cluster). Characterization functional of AcoN-like as negative regulator protein from acetoin cluster in Acinetobacter spp. Strains
Project description:We tested whether home field advantage at inter- and intra-specific levels alters microbial carbon transformations, using a multi-factorial design with microcosms of freshwater submerged leaf litter from two species (Alder and Hemlock) exposed to 'home' or 'away' communities of microbes isolated from decomposing leaf litter, as well as controls with no microbial community added. For all 'home', 'away' and control conditions for each species we also had high or low oxygen treatments. Samples were taken at day 0, 154, 257 and 354 and extracted with solid phase columns and methanol to provide extracted metabolites.
Project description:Aerobic biodegradation in aquatic and marine environments of textile microfibers released to the environment during home laundering.
