Project description:Analysis of gene changes in different genes modulation in MSCs and compared to primary human osteosarcoma cells Total RNA isolated from human parental MSCs and from MSCs siRB, OeMyc, SiRb-OeMyc and primary human osteosarcoma cells
Project description:Analysis of gene expression profile at different developmental stages of MSCs. iPS cells from human fibroblasts are differentiated into MSCs undergoing primitive streak and mesoderm. Results provide important information of dynamic changes in gene expression profile during MSC development.
Project description:Early osteoinductive bone marrow MSCs (e-MSCs) acquire enhanced hematopoiesis-supportive ability. We performed microarray analysis on e-MSCs. Cell chemotaxis-assosiated genes were positively enriched and cell adhesion-associated genes were negatively enriched compared with control MSCs. The expression of CXCL12 and VCAM1 extremely decreased.
Project description:Several studies have suggested that MSCs have pleiotropic immuno-modulatory effects, including inhibition of T cell proliferation, suppression of NK cells proliferation, modulation of cytokine production, and inhibition of dendritic cell (DC) maturation etc. But the exactly mechanism are still largely unclear.We have found that the gene expression profile of psoriatic dermal MSCs was distinct different from normal MSCs. We proposed to investigate the DNA methylation profile of psoriatic MSCs.