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The impact of whole glucan particles (WGP) treatment on pancreatic myeloid cells

ABSTRACT: The impact of whole glucan particles (WGP) treatment on pancreatic myeloid cells

PROVIDER: PRJNA777580 | ENA |

REPOSITORIES: ENA

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The impact of whole glucan particles (WGP) treatment on pancreatic myeloid cells

Project description:Despite the remarkable success of immunotherapy in many types of cancer, pancreatic cancer has yet to benefit. Innate immune cells are critical players in antitumor immunosurveillance. Recent studies have revealed that innate immune populations may possess a form of memory, termed trained immunity through transcriptomic, epigenetic, and metabolic reprograming. 1Here, we show that intraperitoneal administration of yeast-derived particulate β-glucan results in 90% of that dose trafficking to the pancreas. This trafficking leads to a robust influx of newly characterized innate immune cells with an inflammatory monocyte/macrophage phenotype into the pancreas in a CCR2 dependent manner. These cells also exhibit a trained immunity phenotype upon exposure to tumor-derived factors and show enhanced phagocytosis and ROS-mediated cytotoxicity against pancreatic tumors. In orthotopic models of pancreatic cancer, mice trained with β-glucan show significantly reduced tumor burden and prolonged survival, which is further enhanced when combined with anti-PD-L1 immunotherapy. Cumulatively, these findings highlight a novel trafficking mechanism of yeast-derived particulate β-glucan that incites a phenotype of trained immunity specifically in the pancreas that can be utilized as an innovative strategy to treat pancreatic cancer.

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The effect of whole-glucan particles on the gene expression of polarized M2 bone-derived macrophages

Project description:Bone-marrow macrophages polarized to M2 phenotype are immunosuppressive. Interestingly, treatment with whole-glucan particles converts M2 macrophages to M1 phenotype with an anti-tumor phenotype. In this study, the effect of WGP treatment for 6 hours on the gene expression of M2 macrophages was assessed.

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The role of HIF-1 in beta-glucan induced response in myeloid cell

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2014-09-26 | E-GEOD-57206 | biostudies-arrayexpress

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The role of HIF-1 in beta-glucan induced response in myeloid cell

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The effect of beta-glucan - induced trained immunity on the transcriptomic profile of bone marrow granulocyte-macrophage progenitors (GMP)

Project description:Induction of trained immunity by beta-glucan affects myeloid cells and their bone marrow progenitors. In particular, broad alterations in the transcriptome of trained myeloid cells have been demonstrated. In this study, we performed RNA sequencing in GMP from beta-glucan treated mice, as compared to control-treated mice, in order to investigate the impact of trained immunity on the transcriptomic profile of GMP.

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The effect of beta-glucan - induced trained immunity on the epigenetic profile of bone marrow granulocyte-macrophage progenitors (GMP) and neutrophils

Project description:Induction of trained immunity by beta-glucan affects myeloid cells and their bone marrow progenitors. In particular, broad epigenetic alterations in trained myeloid cells have been demonstrated. In this study, we performed single cell ATAC sequencing in GMP and neutrophils from beta-glucan treated mice, as compared to control-treated mice, in order to investigate the impact of trained immunity on the epigenetic profile of GMP and neutrophils.

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Genome-wide analysis of mDC transcriptional response to cyclic glucan

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miRNA expression in myeloid-derived suppressor cells (MDSCs) treated with or without whole ?-glucan particles (WGP)

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