Project description:To investigate the effect of PL on gene expression, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to suppress the cell proliferation of OSCC cell lines. SAS and HSC-3 cells were treated with PL (5 μmol/L) for 4 h in vitro.
Project description:The study aimed at finding the molecular mechanism of action of PL and AP in breast cancer cells. The dataset shows the comparison of gene expression in Plumbagin/Acetyl Plymbagin treated MCF-7 cells when compared with untreated samples.
Project description:To investigate the effect of EGFR disruption on gene expression, we generated EGFR-knockout (EGFR-KO) cell clones using CRISPR/Cas9 system with OSCC cell lines HSC-2 and HSC-3 cells. The OSCC cells were incubated for 48 h in vitro.
Project description:To investigate the effect of PBK inhibitor OTS514 on gene expression, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to suppress the cell proliferation of human OSCC cell lines. The OSCC cells were treated with OTS514 (20 nmol/L) for 24 h in vitro.
Project description:The study aimed at finding the molecular mechanism of action of PL and AP in breast cancer cells. The dataset shows the comparison of gene expression in Plumbagin/Acetyl Plymbagin treated MCF-7 cells when compared with untreated samples. 11 samples were analyzed. MCF-7 cells treated with 10 uM PL or AP for 6 hours. Please note that three samples were done in triplicates and one sample MCF-7 treated with 10uM PL was done in duplicates as one of the replicate RNA didn't show up a good RIN number, SAMPLE1 &2 are replicates, SAMPLE 3,4 &5 are triplicates, SAMPLE 6,7 & 8 are triplicates and SAMPLE 9,10 & 11 are triplicates (SAMPLE numbers are indicated in the description field).
Project description:E. coli MG1655 was grown in the presence of either PL (Plumbagin)/JU (Juglone)/ME (Menadione) (50 micro molar) and DMSO (0.75%)as control, and subjected to microarray analysis. The aim of the work was to understand the structure-function relationship between plumbagin and its analogues.
Project description:Oral squamous cell carcinoma (OSCC) is a lethal disease and early death usually occurs as a result of local invasion and regional lymph node metastases. We used microarrays to identify down or upregulated genes in OSCCs compared with non-malignant controls. To identify genes whose transcription is deregulated in OSCC, the gene expression profiles of eight OSCC cell lines (H-series and M9) and three primary cultures of normal oral keratinocytes (NKs) were examined using Affymetrix HG-U133A and HG-U133 Plus 2.0 arrays.
Project description:Differentially expressed genes between T4 and T1 stage human oral squamous cell carcinoma (OSCC) 36 OSCC samples separated by tumor size T stage, in dual color microarray, using a pool of human cell lines as control. Screening for differentially expressed genes was performed in a cohort of 36 patients randomly selected. Transcriptional profiles were performed using Agilent one color hybridizations in a G4851A platform.