Project description:Comparison of transcriptome of 24 hours post fertilisation wild-type zebrafish embryos and that of embryos depleted of Kcng4b using anti-Kcng4b CRISPR (5’- GGCAGAGAAGGAATTCAGCA-3’, gRNA target site in exon 3) and splice site-blocking MO (5′-TGCATTCGCCCTGTAAAAGAACAAA-3′) targeting kcng4b intron 2-exon 3 (I2E3) junction.
Project description:In this experiment, we've examined chromatin conformation of zebrafish embryos at 24 and 48 hours post-fertilization (hpf), as well as 48 hpf fibroblasts. The aim of this study was to characterise the 3D chromatin structure of zebrafish and perform an evolutionary comparison with mammalian genomes (Homo sapiens and Mus musculus) focusing on syntenic regions and zebrafish ohnologs (duplicated genes that were kept in the genome after the third round of whole-genome duplication).
Project description:We used 10X Genomics Chromium system to profile single cells from a pool of Wild-type Ciona robusta embyros at 12 hours post fertilization (hpf)
Project description:Transcriptomic profiling of the response to dioxin in developing zebrafish embryos, at 24, 48, 72, 96, and 120 h post-fertilization. The goal was to elucidate mechanisms by which dioxin causes toxicity in zebrafish; this dose was previously shown to induce teratogenesis.