Ontology highlight
ABSTRACT:
PROVIDER: PRJNA841353 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We identified a synthetic siRNA (B11) that improves the growth of primary human fibroblasts derived from Friedreich ataxia (FA) patient. A control siRNA, in which one base pair of B11 is mutated (Mut1), was associated with a partial loss of phenotype. This experiment compares the transcriptome of primary FA fibroblasts transfected with B11 siRNA versus the transcriptome of FA cells transfected with Mut1.
2018-11-21 | E-MTAB-4699 | biostudies-arrayexpress
Project description:WT M. abscessus ATCC_19977 was used to construct a targeted deletion mutant lacking the sRNA B11 and a complemented version of the mutant with ectopic expression of B11 from its native promoter at the L5 site. The deletion mutant was also complemented with versions of B11 that lacked a single C from the first loop or had an extra C in the second loop. All strains were grown to log phase and the extracted RNA was used to perform expression profiling by RNAseq.
2022-10-05 | GSE214640 | GEO
Project description:gnp3_tri33-arabidoseed - seed development - WP3 : Biodiversity of seed traits : state of the art - This experiment is a time course performed to choose the best harvesting point to maximise the number of detectable transcripts (8, 10, 12 and 14 dap). Keywords: time course
2009-05-30 | GSE16242 | GEO
Project description:Understanding the regulatory roles of small RNAs (sRNAs) in Mycobacterium marinum is crucial for elucidating its pathogenesis. Here, we present transcriptome profiles of M. marinum strains with deletions and completions of sRNA B11. Through RNA sequencing analysis, we identified significant alterations in gene expression patterns between the B11-deleted and completed strains.
2024-05-01 | GSE264453 | GEO
Project description:gnp3_tri33-arabidoseed - seed specific genes - WP3 : Biodiversity of seed traits : state of the art - This experiment aimed to identify the genes expressed in the seeds vs siliques (easiest to harvest), and to know if the expression pattern of the whole silique could provide a valuable information. Keywords: organ comparison
2009-05-30 | GSE16244 | GEO
Project description:Salmonella remains an important enteric pathogen of poultry, primarily due to concerns regarding food-borne illness in humans consuming contaminated poultry products. Specific probiotic cultures are efficacious as a treatment for neonatal poultry to prevent enteric infections (competitive exclusion) due to the exquisite susceptibility of young chicks to pathogens in the hatchery and brooding environment. The objective of this experiment was to analyze transcriptional profiles in the ceca of neonatal chicks using the Arizona Gallus gallus 20.7K Oligo Array v1.0, following treatment with a probiotic culture derived from poultry with and without Salmonella enterica subsp. Enteritidis (SE) challenge. Chicks were obtained from a commercial hatchery, and challenged with SE upon arrival at the laboratory. One hour post-challenge, chicks were treated with a probiotic culture (FM-B11). Treatment groups included: Control (no challenge or treatment, vehicle only), SE (challenged only), B11 (treated only) and SE+B11 (challenged and treated). Samples were obtained at 12h and 24h post-treatment. We observed that administration of a Lactobacillus-based probiotic culture to chicks following challenge with SE reduced SE colonization of the cecae and resulted in differential expression of genes in the cecae. Among all four treatment groups, 309 genes were differentially expressed (p<0.05) at 12h, and 352 genes were differentially expressed (p<0.05) at 24h. Keywords: disease state analysis
2010-01-14 | GSE19887 | GEO
Project description:gnp3_tri33-arabidoseed - preliminary experiment:repetability - WP3 : Biodiversity of seed traits : state of the art - The third experiment is a comparison of the transcriptome of two parents (Bay0,Sha) and two RILs (104, 140) in loop experimental design. Plants are grown in individual pots on a tray.Flowers were tagged on the flowering day using colored tape. Siliques were harvested 10 days after flowering, freezed in liquid nitrogene and stored at -80degreeC until RNA extraction. Keywords: genotype and ecotype comparison
2009-05-30 | GSE16241 | GEO
Project description:Peptide products from individual cleavage assays with the OOP and PreP1 enzymes were acidified by formic acid and injected into LC-MS. 2012oct12 experiment - sample labels (A1, A2, A3, B1, B2, etc...) condition: 1 - OOP 2 - PreP1 3 - ctrl peptide: A - F1beta H-1 4-15 B - F1beta H-2 43-53 C - F1beta 2-20 D - L29 E - CysD F - SytII G - Mdh oct H - Sdh oct I - P1 J - P22 K - Cox4-preseq L - Abeta28 2013jul12 experiment - sample labels (A11, A21, A31, B11, B21, etc...) condition: 1 - ctrl 2 - OOP 3 - PreP1 peptide: A - thrRS-1 B - thrRS-2 C - F1b-H2 D - L29 E - AT5G36880-11 F - F1b-53 G - AT5G36880-19 H - AT5G36880-26 I - Abeta40
2013-09-18 | PXD000444 | Pride
Project description:Mammuthus mitogenome targeted enrichment experiment
| PRJNA203140 | ENA
Project description:To study the role of NRAS mutations in cell proliferation and self-renewal in acute myeloid leukemia (AML), the human AML cell line, THP1, was modified to replace its naturally occurring heterozygous NRAS-G12D mutation with a doxycycline(dox)-inducible heterozygous NRAS-G12V mutation. The endogenous copies of the NRAS-G12D allele were deleted using CRISPR/Cas9 after a dox-inducible, CRISPR resistant, NRAS-G12V transgene was introduced into the THP1 cell line. The resulting cell line was named B11. RNA-seq data confirmed that endogenous NRAS G12D was successfully replaced by dox-inducible exogenous NRAS G12V in the B11 cell line. As expected, depletion of dox induced G1 cell cycle arrest. Interestingly, the B11 cells experienced ten-times higher expression of NRAS induced G2/S-phase cell cycle arrest. Forty-nine genes were identified as signaling responsible genes associated with high expression of NRAS.
2022-07-25 | GSE115911 | GEO