Project description:We utilized single cell-indexed custom RNA-sequencing to interogate the transcriptomes of isotype specific plasma cells at homeostasis and following immunization with a model antigen
Project description:Bone marrow long-lived plasma cells are essential for long-term protection against infection and their persistence within this organ relies on interactions with Cxcl12-expressing stromal cells that are still not clearly identified. Here, using single cell RNAseq and in silico transinteractome analyses we identified LepR+ mesenchymal cells as the stromal cell subset most likely to interact with plasma cells within the bone marrow. Moreover, we demonstrated that depending on the isotype they express, plasma cells may use different set of integrins and adhesion molecules to interact with these stromal cells. Altogether, our results constitute an unprecedented characterization of plasma cell subset stromal niches and open new avenues for the specific targeting of bone marrow plasma cells based on their isotype.
Project description:Plasma cell gene expression is driven both by isotype and tissue location. In this series we examine gene expression of bone marrow IgA, IgM and IgG plasma cells as well as IgA plasma cells from small intestine lamina propria. To validate tissue specific gene expression we also include gene expression from lamina propria IgA-/- plasma cells. All plasma cell samples are from Blimp1+/GFP reporter animals and splenic follicular and marginal zone B cell gene expression have been added as reference populations.
Project description:In this study we identify SUMO isotype-specific non covalent binders. The different isotypes include SUMO1, SUMO2 and a 3xSUMO2 chain.
Project description:Determine irf4 target genes in mouse B cells undergoing LPS differentiation to plasma cells in vitro. Determine targets of IRF4 by overexpression in a human GCB cell line. Keywords: cell type comparison design