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Proteomic profiling of xenobiotic and nutrient transporters in human placenta of different gestational ages

ABSTRACT: The placenta serves a critical role in nutrient uptake and waste elimination for the developing fetus and is responsible for the uptake and/or exchange of xenobiotics, including medications, between the maternal and fetal bloodstreams. Very little is understood about fetal drug and nutrient exposure during pregnancy and how it may change over the course of fetal development. The primary goal of this work was to profile the abundance of placental xenobiotic and nutrient transporters over the course of gestation using a data-independent analysis (DIA) proteomics approach. Building a deeper understanding of these proteins at the interface between the fetal and maternal bloodstreams will inform future studies designed to address questions regarding the safety and efficacy of therapeutics and nutrient supplements used during pregnancy. A total of 139 solute carrier (SLC) and ATP-cassette binding (ABC) transport proteins were identified across all samples, 80 of which were found in every sample (n = 34). Seventeen transport proteins (14 SLC, 3 ABC) were found to be significantly altered between trimesters during development. To the best of our knowledge, this study represents the first global proteomic characterization of placental membrane-associated proteins using DIA-MS proteomics and lays the groundwork for further advancements in the understanding of the membrane-associated proteome during fetal development.

ORGANISM(S): Homo Sapiens

SUBMITTER: Eric Weaver

PROVIDER: PXD080148 | panorama | Thu Jun 25 00:00:00 BST 2026

REPOSITORIES: PanoramaPublic

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Project description:To investigate global changes in gene expression during B. emersonii germination process, we constructed cDNA microarrays containing 3,563 putative unique genes. Analyses were carried out during germination induced under various environmental conditions. Microarray data revealed that 26% of them are differentially expressed during germination in nutrient medium, in at least one of the time points investigated. Over 500 genes are up regulated during the time course of germination under these conditions, most of them being related to cell growth, including genes involved in protein biosynthesis, DNA transcription, energetic metabolism, carbohydrate and oligopeptide transport, and cell cycle control. On the other hand, several transcripts stored in the zoospores are down regulated during germination in nutrient medium, such as genes involved in signal transduction, amino acid transport and chromosome organization. We also compared germination induced in the presence of nutrients with that triggered either by adenine or potassium ions in inorganic salt solution. Several genes involved in cell growth that are induced during germination in nutrient medium do not show increased expression when B. emersonii zoospores germinate in inorganic solution, indicating that nutrients exert a positive regulatory effect on the transcription of these genes. Differentially expressed genes during germination were investigated in a time-course experiment, using RNA samples isolated from cells collected 30, 60 and 90 min after inoculation of zoospores in nutrient medium DM3. The reference sample was zoospore RNA, which was considered the time zero of germination. We also compared germination in nutrient medium with germination induced in inorganic salt solution, triggered either by adenine or potassium ions. In these experiments we considered germination in nutrient medium as the reference sample. There are at least 3 biological replicates (independent harvest) and 2 technical replicates for each array (L-left and R-right).

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Proteomic profiling of xenobiotic and nutrient transporters in human placenta of different gestational ages

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