Project description:This dataset contains mass spectrometry-based proteomics data from subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and brown adipose tissue (BAT) isolated from wild-type and adipose-tissue-specific myoglobin knockout (ATMBKO) mice maintained at room temperature (23°C), thermoneutrality (30°C), or exposed to cold (8°C). The objective of the study was to determine the contribution of adipose myoglobin to thermogenesis and thermoregulation. The dataset provides a resource to investigate depot-specific and temperature-dependent proteomic changes associated with adipose myoglobin deficiency.
Project description:This study investigated longitudinal changes in adipose tissue–derived extracellular vesicle protein cargo, referred to as adiposomes, following bariatric surgery in adults with obesity. 23 adults with obesity scheduled for sleeve gastrectomy were enrolled. Subcutaneous adipose tissue samples and clinical cardiometabolic measurements were collected before surgery and again approximately 12–14 weeks after surgery.
Project description:We collected adipose tissue specimens during elective surgery (inguinal hernia, cryptorchism). Samples were proceesed for extraction of total RNA, using phenol:chlorophorm extraction. Next generation RNA sequencing were performed. Published article: https://www.mdpi.com/1422-0067/24/23/16706
Project description:Interactions between stromal cell-derived factor-1M-NM-1 (SDF-1M-NM-1) and its cognate receptor CXCR4 are crucial for the recruitment of mesenchymal stem cells (MSCs) from bone marrow (BM) reservoirs to damaged tissues for repair during alarm situations. MicroRNAs are differentially expressed in stem cell niches, suggesting a specialized role in stem cell regulation. Here, we gain insight into the molecular mechanisms involved in regulating SDF-1M-NM-1. Individualized outcome prediction classifiers were successfully constructed through expression profiling of microRNAs (in all organisms as annotated in Sanger miRBase Release 11.0 (http://microrna.sanger.ac.uk))in one burned murine skin tissue compared to normal skin tissue,which had 57 upregulated microRNAs and 28 down-regulated microRNAs. In the study presented here, a consecutively operated, well-defined cohort of 2 cases, was used to acquire expression profiles of microRNAs (in all organisms as annotated in Sanger miRBase Release 11.0 (http://microrna.sanger.ac.uk)), leading to the successful construction of supervised. The most recent version of the array (v.11.0 - hsa, mmu & rno array) contains more than 1700 capture probes, covering all microRNAs annotated in miRBase 11.0, as well as all viral microRNAs, related to these species.