Project description:A control group of male C57BL/6J mice were fed a 60% fat diet (Research Diets D12492i) for 8 weeks, resulting in “ad libitum obesity” (ALO). Leptin receptor-deficient B6.BKS(D)-Leprdb/J (“db/db”) male mice were fed a low-fat chow diet (PicoLab Rodent Diet 20; Purina Mills Inc). Once the average body weights of the two groups were equivalent, all mice were fasted for 24 hours, after which they were sacrificed and perigonadal adipose tissue (PGAT) was dissected out. PGAT was cultured ex vivo for 6 hours, after which conditioned media was collected and submitted for proteomic profiling. At the time of sacrifice, ALO mice were 17 weeks old and db/db mice were 10 weeks old. “HFD” is also used to refer to the ALO condition while “DB” is used to refer to the “db/db” condition.
Project description:Male C57BL/6J mice were implanted with intragastric catheters that allowed direct infusion of liquid into the stomach. A control group of mice received saline through the catheter and was given free access to high-fat diet (Research Diets D12492i, 60% calories from fat) over a span of months, leading to “ad libitum obesity” (ALO). Another group of mice received a hypercaloric liquid diet through the catheter, leading to an acute-onset, “overfeeding-induced obesity” (OIO). OIO mice were also given free access to high-fat diet but did not consume it while receiving hypercaloric intragastric infusions. After 12 days of infusions, the average weights of the two groups were equal. All mice were fasted for 24 hours, after which they were sacrificed and perigonadal adipose tissue (PGAT) was dissected out. PGAT was cultured ex vivo for 6 hours, after which conditioned media was collected and submitted for proteomic profiling. All mice were age-matched and 16 weeks old at time of sacrifice. “HFD” is also used to refer to the ALO condition, while “OID” is also used to refer to the OIO condition.
Project description:Cold exposure imposes a metabolic challenge to mammals that must be met by a coordinated repsonse in different tissues to prevent hypothermia. This study reports analysis of transcriptome profiles in brown adipose tissue, liver, white adipose of mice in repsonse to 24 hour cold exposure