Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Liver_Mito_UT

ABSTRACT: Liver Mitochondrial Fraction - UT

INSTRUMENT(S): LCQ DECA XP

ORGANISM(S): Mus Musculus

SUBMITTER: Andrew Emili

PROVIDER: PAe000369 | PeptideAtlas |

REPOSITORIES: PeptideAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PAe000369_4958_search.params.tar.gz	Other
	PAe000369_4958_searchdatabase_tandem.fasta	Fasta
	PAe000369_4958_tandem.params	Other
	PAe000369_RAW_201104191048.tar.gz	Other
	PAe000369_README	Other

Items per page:

1 - 5 of 11

Similar Datasets

Identification of the UT-A1 Urea Channel Interactome

Project description:The urea channel Slc14a2 (or UT-A1) mediates vasopressin-regulated urea transport across the inner medullary collecting duct (IMCD). Previously, UT-A1 was found to present in a high molecular weight complex, suggesting UT-A1 is involved in certain protein-protein interactions. The present study sought to identify the proteins that interact with UT-A1 in this complex for a better understanding of how UT-A1 is regulated. Rat IMCD suspensions were treated with or without V2 receptor agonist, dDAVP, followed by in-cell crosslinking using BSOCOES and detergent solubilization. Immunoprecipitation using Dynabeads coated with UT-A1 specific antibody successfully pulled down the UT-A1 proteins. In-gel digestion protocol was carried out to prepare samples for liquid chromatographic mass spectrometry analysis of tryptic peptides using a Velos-Orbitrap mass spectrometer. The peptides passing stringent spectral quality thresholds were quantified (label-free) to identify those with (UTA-1 antibody/preimmune IgG) >4. A total of 128 UT-A1 interacting proteins were identified. Gene Ontology analysis maps the distribution of these proteins throughout major cell compartments: endoplasmic reticulum, Golgi, endosomes, cytosol and plasma membrane. Among them are four protein kinases (Cdc42bpb, Phkb, Camk2d, Mtor) that play roles in vasopressin-regulated phosphorylation of UT-A1. Non-label quantification was also performed to determine the stoichiometry of UT-A3 with UT-A1, the result does not support an oligomeric complex formation of UT-A1/A3. In conclusion, we have provided a refined list of UT-A1 binding proteins which can be useful for further analysis of the vasopressin signaling pathway in regulation of UT-A1 in IMCD.

2019-11-08 | PXD003496 | Pride

Heart_Mito_UT

Project description:Heart Mitochondrial Fraction - UT

| PAe000353 | PeptideAtlas

Placenta_Mito_UT

Project description:Placenta Mitochondrial Fraction - UT

| PAe000366 | PeptideAtlas

Brain_Mito_UT

Project description:Brain Mitochondrial Fraction - UT

| PAe000359 | PeptideAtlas

Kidney_Mito_UT

Project description:Kidney Mitochondrial Fraction - UT

| PAe000358 | PeptideAtlas

Lung_Mito_UT

Project description:Lung Mitochondrial Fraction - UT

| PAe000357 | PeptideAtlas

Targeted quantitative analysis of specific proteins in cytosolic, mitochondrial, and nuclear fractions using PRM

Project description:We present a targeted proteomic approach using parallel reaction monitoring (PRM) to assess the enrichment of cytosolic, mitochondrial, and nuclear fractions obtained from liver samples using commercial isolation kits. For mitochondrial fraction, CPS1 and ATP5F1 were chosen. For the cytosolic fraction, HSP90AB1 and AHCY were selected. For the nuclear fraction, LMNA and NPM1 were used as markers.

2026-04-13 | PXD067737 | Pride

Mouse liver LC-MS/MS - The influence of AICAR - direct activator of AMP-activated protein kinase (AMPK) - on liver proteome in apoE-knockout mice

Project description:Shotgun LC-MS/MS on mitochondrial and cytosolic fraction of the liver of apoE-knockout mice

2017-05-02 | PXD002615 | Pride

Autoantibodies secreted by TLR7-stimulated B cell subsets from mice with primary Sjogren's syndrome

Project description:Spleens (n = 2-3) were harvested from mice with primary Sjogren's syndrome (NOD.B10) and cells were sort-purified. Follicular B cells (FO) (6 million), Marginal zone B cells (4.5 million) and age-associated B cells (500,000) were sorted. Cells were cultured in 200 uL of complete RPMI media containing 62.5 ng/mL of imiquimod, a TLR7 agonist. Cells were cultured for 6 days, and the supernatants were harvested and stored at -20 C. Samples were shipped to UT Southwestern for autoantigen array analysis.

2023-10-24 | GSE236255 | GEO

Radiation response in xenografts of the head and neck cancer UT14 cell line

Project description:Low passage head and neck squamous cancer cells (UT-14-SCC) were injected into the flanks of female nu/nu mice to generate xenografts. After tumors reached a size of 500mm3, they were treated with either sham RT or 15 Gy in one fraction. At different time points, days 0, 3, 10 and for controls and days 4, 7, 12, and 21 after irradiation, the tumors were harvested for global gene expression analysis and pathway analysis.

2014-09-19 | GSE61573 | GEO