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MTB_starved_cultureFiltrateSamples

ABSTRACT: Triplicate culture filtrate samples of normal (log phase) and PBS cultured (starvation) M. tuberculosis were separated by SDS-PAGE, and 10 bands were excised from each lane after staining with Coomassie R-250. In-gel trypsin digestion was performed on gel bands followed by nanoLC. Eluting peptides were then analyzed on an LTQ FT instrument.

REANALYSIS of: PXD000111

INSTRUMENT(S): LTQ FT

ORGANISM(S): Mycobacterium Tuberculosis

SUBMITTER: Ida Rosenkrands

PROVIDER: PAe003970 | PeptideAtlas | 2013-12-31

REPOSITORIES: PeptideAtlas

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Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-starvation-responsive toxin-antitoxin systems.

Albrethsen Jakob J Agner Jeppe J Piersma Sander R SR Højrup Peter P Pham Thang V TV Weldingh Karin K Jimenez Connie R CR Andersen Peter P Rosenkrands Ida I

Molecular & cellular proteomics : MCP 20130123 5

In order to successfully enter the latent stage, Mycobacterium tuberculosis must adapt to conditions such as nutrient limitation and hypoxia. In vitro models that mimic latent infection are valuable tools for describing the changes in metabolism that occur when the bacterium exists in a non-growing form. We used two complementary proteomic approaches, label-free LC-MS/MS analysis and two-dimensional difference gel electrophoresis, to determine the proteome profile of extracellular proteins from ...[more]

PMID: 23345537

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Mycobacterium tuberculosis culture filtrate LC-MS/MS

Project description:Triplicate culture filtrate samples of normal (log phase) and PBS cultured (starvation) M. tuberculosis were separated by SDS-PAGE, and 10 bands were excised from each lane after staining with Coomassie R-250. In-gel trypsin digestion was performed on gel bands followed by nanoLC. Eluting peptides were then analyzed on an LTQ FT instrument, and the resulting raw files were searched with Sequest (part of Proteome Discoverer) against an M. tuberculosis H37Rv database (downloaded from the Sanger Centre). The final combined dataset comprised 12,399 unique peptides derived from 1,362 proteins, each identified by a minimum of two distinct peptides.

2013-01-24 | PXD000111 | Pride

Comparison of protein and peptide fractionation approaches in protein identification and quantification from Saccharomyces cerevisiae

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MS analysis of BCG and recombinant BCG "70C-RBD"

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Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP: sequencing data

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Membrane Fraction Complexome of Nitrososphaera viennensis with BN-PAGE and Examination of the Archaeal Ammonia Monooxygenase Complex via SDS-Tricine-PAGE

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Comparison of iPSC-derived alveolar epithelial cell culture methods

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