Browse
Submit Data
Databases
API
Help

Dataset Information

6 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SHR_LysC_Velos

ABSTRACT: SHR_liver, LysC, LTQ Orbi Velos

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Henk van den Toorn

PROVIDER: PAe004335 | PeptideAtlas | 2013-12-31

REPOSITORIES: PeptideAtlas

ACCESS DATA

Json Xml

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

SHR_Chymo_Velos

Project description:SHR_liver, Chymo, LTQ Orbi Velos

2013-12-31 | PAe004334 | PeptideAtlas

SHR_GluC_Velos

Project description:SHR_liver, GluC, LTQ Orbi Velos

2013-12-31 | PAe004337 | PeptideAtlas

SHR_Trypsin_Velos

Project description:SHR_liver, Trypsin, LTQ Orbi Velos

2013-12-31 | PAe004338 | PeptideAtlas

SHR_AspN_Velos

Project description:SHR_liver, AspN, LTQ Orbi Velos

2013-12-31 | PAe004320 | PeptideAtlas

Profiling of HLA-I ligandomes discovers novel cervical cancer-associated antigens

Project description:The product ion scans were acquired with a 2.0-unit isolation width and a normalized collision energy of 35 in an LTQ-Orbitrap Velos Pro MS spectrometer (Orbitrap Velos Pro, Thermo Fisher Scientific, US).

2023-06-05 | PXD042745 | iProX

BN_LysC_Velos

Project description:BN-Lx_liver, LysC, LTQ Orbi Velos

2013-12-31 | PAe004326 | PeptideAtlas

Protein phosphorylation evolution across 18 fungal species

Project description:Protein phosphorylation evolution across 18 fungal species. For each species whole cell extracts were digested separately with trypsin and LysC. Each digest was IMAC enriched and analyzed by LC-MS/MS in technical triplicate on an LTQ Orbitrap Velos using a 120min method. Data was searched against the corresponding species using Sequest/Comet, and filtered to 1% FDR at the PSM level. For S.Cerevisiae, S.Pombe and S.Mikatae, cells were grown in triplicate under 3 different conditions: YPD, YPD + mild temperature stress and optimized "best media". For these cells whole cell extracts were produced, digested with trypsin and IMAC enriched and analyzed by LC-MS/MS on an LTQ Orbitrap Velos using a 120min method. Data was searched against the corresponding species using Sequest/Comet, and filtered to 1% FDR at the PSM level. S. cerevisiae cells transformed with tagged versions of CDC33 WT, alanine (S28A) and glutamic acid (S28E) mutants were generated. Mutant strain were grown three distinct (heavy, medium and light) SILAC media under overexpression conditions. Cells for each construct were harvested and pooled together and used for the pull down experiments, done in triplicate. Pulldown eluate was digested with LysC and analyzed on a QExactive instrument with 90min method. Data was searched against S.Cerevisiae database including the tagged CDC33 proteins using Sequest/Comet, and filtered to 1% FDR at the PSM level.

2015-12-18 | MSV000079428 | MassIVE

Germ-free mouse ileum iTRAQ shotgun LC-MSMS

Project description:Shotgun proteomics of normal versus germ-free mouse terminal ileum. 39 mice. Male and female. BALBc and C57BL10. The samples were labeled using iTRAQ 8plex and analyzed using a nano-LC-LTQ-Orbitrap-Velos-MSMS. This is the proteomics data from an n-omics project.

2017-10-04 | PXD003177 | Pride

Proteomics analysis of B. bigemina

Project description:Protein from B. bigemina PR cell lysates was analysed by 1D SDS PAGE and in solution digestion. Digested peptides were analysed by on-line nanoflow liquid chromatography using the nanoACQUITY-nLC system (Waters MS technologies, Manchester, UK) coupled to an LTQ-Orbitrap Velos (ThermoFisher Scientific, Bremen, Germany). Collected data were then analysed by Mascot (version 2.3.02, Matrix Science) and Progenesis LC–MS (version 4.1, Nonlinear Dynamics, UK).

2014-06-16 | PXD000629 | Pride

Analytical framework for peptidomics applied to large scale neuropeptide identification

Project description:Endogenous peptides are extracted and seperated with nLC with HCD fragmentation on a LTQ Orbitrap Velos. Data analysis was done with Mascot and MaxQuant

2016-05-03 | PXD002431 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data