Project description:The product ion scans were acquired with a 2.0-unit isolation width and a normalized collision energy of 35 in an LTQ-Orbitrap Velos Pro MS spectrometer (Orbitrap Velos Pro, Thermo Fisher Scientific, US).
Project description:Gene expression profiling of human embryonic kidney (HEK293) cells was performed to determine the effect of high and low glucose on gene expression. Microarrays were used to identify distinct classes of genes up-regulated in HEK293 cells during cultivation for 7 days in medium with physiological (low) glucose compared to high glucose. Human embryonic kidney cells (HEK293) were cultivated for 7 days in commercially available DMEM (supplemented with 10 % FCS) containing high glucose (450 mg/dl) or low glucose (100 mg/dl). Cells were harvested, total RNA was extracted and microarray gene expression profiling was performed to compare differential gene expression between low and high glucose conditions. Two biological replicates for each condition were made (high glucose: HEK-high-gluc-1 and HEK-high-gluc-2; and low glucose: HEK-low-gluc-1 and HEK-low-gluc-2).
Project description:Endogenous peptides are extracted and seperated with nLC with HCD fragmentation on a LTQ Orbitrap Velos. Data analysis was done with Mascot and MaxQuant
Project description:21 samples of liver RNA coming from mice treated with glucagon (Gluc), thyroid hormone (T3), both, or the conjugated compound (Cp). Each sample comes from a single mice.