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CIP SILAC GeLC FT MS - Analysis of the membrane proteome of ciprofloxacin-resistant macrophages by stable isotope labeling with amino acids in cell culture (SILAC)

ABSTRACT: SILAC-based GeLC FT-ICR MS approach on Light labeled Ciprofloxacin-resistant J774 macrophage membrane proteome and Heavy labeled WT J774 macrophage membrane proteome (Fraction F2).

INSTRUMENT(S): instrument model, LTQ FT Ultra

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Bart Devreese

PROVIDER: PRD000419 | Pride | 2013-02-19

REPOSITORIES: Pride

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Analysis of the membrane proteome of ciprofloxacin-resistant macrophages by stable isotope labeling with amino acids in cell culture (SILAC).

Caceres Nancy E NE Aerts Maarten M Marquez Béatrice B Mingeot-Leclercq Marie-Paule MP Tulkens Paul M PM Devreese Bart B Van Bambeke Françoise F

PloS one 20130307 3

Overexpression of multidrug transporters is a well-established mechanism of resistance to chemotherapy, but other changes may be co-selected upon exposure to drugs that contribute to resistance. Using a model of J774 macrophages made resistant to the fluoroquinolone antibiotic ciprofloxacin and comparing it with the wild-type parent cell line, we performed a quantitative proteomic analysis using the stable isotope labeling with amino acids in cell culture technology coupled with liquid chromatog ...[more]

PMID: 23505477

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