Dataset Information

DYNENDO - Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry

ABSTRACT: Iron dextran-loaded compartments isolated by magnetic purification

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

SUBMITTER: Agnes JOURNET

PROVIDER: PRD000422 | Pride | 2011-12-05

REPOSITORIES: Pride

ACCESS DATA

Publications

Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.

Journet Agnès A Klein Gérard G Brugière Sabine S Vandenbrouck Yves Y Chapel Agnès A Kieffer Sylvie S Bruley Christophe C Masselon Christophe C Aubry Laurence L

Proteomics 20111220 2

The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis process, we established the inventory of proteins associated with this pathway using mass spectrometry-based proteomics. Using a magnetic purification procedure and high-performance LC-M ...[more]

PMID: 22120990

Similar Datasets

Project description:This a model from the article: Systems analysis of iron metabolism: the network of iron pools and fluxes Tiago JS Lopes, Tatyana Luganskaja, Maja Vujic-Spasic, Matthias W Hentze, Martina U Muckenthaler, Klaus Schumann and Jens G Reich BMC Systems Biology2010, Aug 13;4(1):112. 20704761, Abstract: Background Every cell of the mammalian organism needs iron in numerous oxido-reductive processes as well as for transport and storage of oxygen. The versatility of ionic iron makes it a toxic entity which can catalyze the production of radicals that damage vital membranous and macromolecular assemblies in the cell. The mammalian organism maintains therefore a complex regulatory network of iron uptake, excretion and intra-body distribution. Intracellular regulation in different cell types is intertwined with a global hormonal signaling structure. Iron deficiency as well as excess of iron are frequent and serious human disorders. They can affect every cell, but also the organism as a whole. Results Here, we present a kinematic model of the dynamic system of iron pools and fluxes. It is based on ferrokinetic data and chemical measurements in C57BL6 wild-type mice maintained on iron-deficient, iron-adequate, or iron-loaded diet. The tracer iron levels in major tissues and organs (16 compartment) were followed for 28 days. The evaluation resulted in a whole-body model of fractional clearance rates. The analysis permits calculation of absolute flux rates in the steady-state, of iron distribution into different organs, of tracer-accessible pool sizes and of residence times of iron in the different compartments in response to three states of iron-repletion induced by the dietary regime. Conclusions This mathematical model presents a comprehensive physiological picture of mice under three different diets with varying iron contents. The quantitative results reflect systemic properties of iron metabolism: dynamic closedness, hierarchy of time scales, switch-over response and dynamics of iron storage in parenchymal organs. Therefore, we could assess which parameters will change under dietary perturbations and study in quantitative terms when those changes take place. This model corresponds to the Iron Loaded condition - Mice This model originates from BioModels Database: A Database of Annotated Published Models. It is copyright (c) 2005-2010 The BioModels Team.For more information see the terms of use.To cite BioModels Database, please use Le Novère N., Bornstein B., Broicher A., Courtot M., Donizelli M., Dharuri H., Li L., Sauro H., Schilstra M., Shapiro B., Snoep J.L., Hucka M. (2006) BioModels Database: A Free, Centralized Database of Curated, Published, Quantitative Kinetic Models of Biochemical and Cellular Systems Nucleic Acids Res., 34: D689-D691.

Dataset Information

DYNENDO - Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry

Publications

Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure