Proteomics

Dataset Information

0

DYNENDO - Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry


ABSTRACT: Iron dextran-loaded compartments isolated by magnetic purification

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

SUBMITTER: Agnes JOURNET  

PROVIDER: PRD000422 | Pride | 2011-12-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PRIDE_Exp_Complete_Ac_19115.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_19115.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_19115.xml.gz Xml
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Publications

Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.

Journet Agnès A   Klein Gérard G   Brugière Sabine S   Vandenbrouck Yves Y   Chapel Agnès A   Kieffer Sylvie S   Bruley Christophe C   Masselon Christophe C   Aubry Laurence L  

Proteomics 20111220 2


The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis process, we established the inventory of proteins associated with this pathway using mass spectrometry-based proteomics. Using a magnetic purification procedure and high-performance LC-M  ...[more]

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