Dataset Information

Shotgun Proteomics of Brachylophosaurus canadensis Blood Vessels

ABSTRACT: Brachylophosaurus canadensis peptides from blood vessels isolated from within the bone were characterized using high resolution mass spectrometry. This allowed identification of actin, alpha and beta tubulin, various histones, myosin, and tropomyosin. Within the peptides, evidence of age (e.g., deamidation, oxidation, protein backbone cleavage) was present.

INSTRUMENT(S): Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, LTQ Orbitrap XL, LTQ Orbitrap Elite

ORGANISM(S): Gallus Gallus (chicken) Struthio Camelus (ostrich) Brachylophosaurus Canadensis (campanian Hadrosaur)

TISSUE(S): Blood Vessel

SUBMITTER: Leonid Zamdborg

LAB HEAD: Timothy Paul Cleland

PROVIDER: PXD001738 | Pride | 2015-11-30

REPOSITORIES: Pride

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Publications

Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis.

Cleland Timothy P TP Schroeter Elena R ER Zamdborg Leonid L Zheng Wenxia W Lee Ji Eun JE Tran John C JC Bern Marshall M Duncan Michael B MB Lebleu Valerie S VS Ahlf Dorothy R DR Thomas Paul M PM Kalluri Raghu R Kelleher Neil L NL Schweitzer Mary H MH

Journal of proteome research 20151123 12

Structures similar to blood vessels in location, morphology, flexibility, and transparency have been recovered after demineralization of multiple dinosaur cortical bone fragments from multiple specimens, some of which are as old as 80 Ma. These structures were hypothesized to be either endogenous to the bone (i.e., of vascular origin) or the result of biofilm colonizing the empty osteonal network after degradation of original organic components. Here, we test the hypothesis that these structures ...[more]

PMID: 26595531

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Project description:Background: Solubilized aluminum (Al) is rhizotoxic and can be present in acid soils at levels that inhibit root growth. Acid soils are found throughout the world and often support forests. However, unlike in herbaceous plants, little is known about the mechanisms by which forest trees respond to and tolerate Al. To begin to elucidate these mechanisms, we characterized transcriptomic changes in response to Al in roots of aspen (Populus tremula L.). Results: Aspen roots were treated with Al in solution culture for 6 h, 54 h, and 246 h. Transcriptomic changes were assessed by the Affymetrix GeneChip Poplar Genome Array. The analysis revealed 176 induced and 66 suppressed genes. The majority of these genes were regulated at 6 h, presumably reflecting root growth, which was strongly inhibited at 6 h and partially was recovered at 2 d and 10 d. Enrichment analysis identified sets of functionally related genes whose members were statistically over-represented compared to the genes on the microarray. These sets included genes related to cell wall modification, oxidative stress, cell death, and transport processes. Two of the genes involved in transport were related to the Arabidopsis Al tolerance genes AtALS3 (aluminum sensitive 3), possibly mediating redistribution of Al, and AtMATE (multi-drug and toxin extrusion), facilitating exudation of citrate. Expression patterns in response to Al in different plant tissues indicated that the two aspen genes are homologs of AtALS3 and AtMATE, suggesting that aspen and Arabidopsis share common mechanisms to cope with Al. Conclusion: This is the first survey of transcriptomic changes in response to Al in a forest tree. The results of our study provide a valuable set of data, which will help to further our understanding of the mechanisms and their regulation that enable aspen to grow in environments with toxic levels of Al. Aspen roots were treated with Al in solution culture for 6 h, 54 h, 246 h to cover a broad range of exposure times. Transcriptomic changes were assessed by the Affymetrix GeneChip Poplar Genome Array. For each time point, root tips of three independant plants were analyzed.

Dataset Information

Shotgun Proteomics of Brachylophosaurus canadensis Blood Vessels

Publications

Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis.

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