Dataset Information

Schistosoma japonicum cercaria and schistosomula

ABSTRACT: Schistosomiasis is a parasitic zoonosis which caused by schistosomes and poses great threat to the health of human and other mammals. Schistosome cercaria must penetrate skin as an initial step to successfully infect the final host. Proteolytic enzymes secreted from the acetabular glands of cercaria contribute significantly to the invasion process. In this study, we designed a new cercaria transformation experiment to get the penetrated cercaria (schistosomula), then a gel-free shotgun proteomic analysis were performed to compare the proteomes of cercaria and schistosomula. 1894 and 1002 proteins were identified respectively, 924 proteins were common in both of two samples, almost take up 92% of the total proteins of schistosomula. The identified proteins were closely associated with nine main biological processes through Gene Ontology (GO) categorical analysis. The identification of the proteins potentially related to skin penetration offer a global overview of changes during cercaria skin invasion, providing clues into how they involve in the penetration process. Various proteases and peptidases were detected, and were differentially existed before and after cercaria transformation, suggesting proteases-specific roles and complexity mechanism during invasion.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

DISEASE(S): Intestinal Schistosomiasis

SUBMITTER: Mu Liu

LAB HEAD: Mu Liu

PROVIDER: PXD002185 | Pride | 2017-05-08

REPOSITORIES: Pride

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Publications

Proteomic Analysis on Cercariae and Schistosomula in Reference to Potential Proteases Involved in Host Invasion of Schistosoma japonicum Larvae.

Liu Mu M Ju Chuan C Du Xiao-Feng XF Shen Hai-Mo HM Wang Ji-Peng JP Li Jian J Zhang Xu-Min XM Feng Zheng Z Hu Wei W

Journal of proteome research 20150929 11

Schistosomiasis is a parasitic zoonosis posing great threat to human health. The infection is acquired by larval cercariae penetrating host skin and transforming into juveniles, schistosomula. Proteolytic enzymes secreted from the cercarial acetabular glands are known to aid to the skin penetration, but molecular mechanisms remain largely unclear. To profile the protein composition and identify potential invasive proteases, we developed a new method for simulating cercarial transformation and co ...[more]

PMID: 26370134

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Project description:Protein variation in blood-dwelling schistosome worms generated by differential splicing of micro-exon gene transcripts. The infective schistosome cercaria develops from an undifferentiated germ ball within the daughter sporocyst in the molluscan host, during which considerable morphological development and synthesis of proteins essential for infection occurs. The free-living, non-feeding cercaria is notable for its swimming and host location behaviour. On contact with host skin it rapidly penetrates, replaces its tegument surface membranes, and begins body remodelling, before crossing the dermis to exit the skin via a blood vessel. Such a ‘violent’ transition from snail to fresh water to mammalian host should be accompanied by remarkable changes in the patterns of gene expression. All gene models from version E of the S. mansoni genome (www.GeneDB.org) were incorporated into a 350K feature Roche-NimbleGen, high-density oligonucleotide array. From a map-ordered list, every 13th 50mer was chosen as a probe. Double-stranded cDNA from three biological replicates each of germ balls, cercariae, and day 3 schistosomula was hybridised to the array without amplification. Statistical analysis was carried out using programmes from the Bioconductor suite (Gentleman et al. 2004 Genome Biol 5(10):R80). More than 1000 loci were shown to be differentially expressed in each of the comparisons between the three life cycle stages. Gene ontology (GO) analysis was then carried out to discover the categories enriched in each stage. In addition custom categories based on biological function (e.g. stress response) or parasite tissue (e.g. tegument) were analysed. Three biological replicates each of germ balls, cercariae, day 3 in vitro cultured schistosomula, and eggs were used. No technical replicates were included. This is the first genome-wide microarray platform for S. mansoni. It was used to investigate the gene expression patterns of the above lifecycle stages. The first three are the stages before, during and after penetration of mammalian host skin.

Dataset Information

Schistosoma japonicum cercaria and schistosomula

Publications

Proteomic Analysis on Cercariae and Schistosomula in Reference to Potential Proteases Involved in Host Invasion of Schistosoma japonicum Larvae.

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