Zea mays AM fungi interaction - A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane

ABSTRACT: We describe a method that detects membrane proteins from cell type-specific plant plasma membrane subdomains. As the biochemical properties of membrane subdomains are often unknown, general membrane enrichment by continuous sucrose density gradient centrifugation was combined with filter-aided sample preparation, strong cation exchange pre-fractionation and sensitive LTQ Orbitrap Velos-based mass-spectrometry proteomics. This workflow identified plasma membrane proteins, including underrepresented proteins, at an unprecedented level of sensitivity and reproducibility.

INSTRUMENT(S):

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Root

SUBMITTER: Marco Chiapello  

LAB HEAD: Uta Paszkowski

PROVIDER: PXD002575 | Pride | 2018-09-24

REPOSITORIES: Pride