Dataset Information

Proteome profiling of Rhodobacter capsulatus wild type and a ΔmodABC mutant in response to molybdenum

ABSTRACT: The diazotrophic bacterium Rhodobacter capsulatus is able to synthesize two nitrogenases, a molybdenum-dependent and an alternative Mo-free iron-only nitrogenase, enabling growth with molecular dinitrogen (N2) as sole nitrogen source. The Mo response of the wild type and a mutant lacking the high-affinity molybdate transporter, ModABC, were analyzed by proteome profiling.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Rhodobacter Capsulatus (strain Atcc Baa-309 / Nbrc 16581 / Sb1003)

TISSUE(S): Nitrogen Fixing Cell

DISEASE(S): Disease Free

SUBMITTER: Sina Schäkermann

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD003123 | Pride | 2015-12-14

REPOSITORIES: Pride

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Publications

Proteome Profiling of the Rhodobacter capsulatus Molybdenum Response Reveals a Role of IscN in Nitrogen Fixation by Fe-Nitrogenase.

Hoffmann Marie-Christine MC Wagner Eva E Langklotz Sina S Pfänder Yvonne Y Hött Sina S Bandow Julia E JE Masepohl Bernd B

Journal of bacteriology 20151207 4

<h4>Unlabelled</h4>Rhodobacter capsulatus is capable of synthesizing two nitrogenases, a molybdenum-dependent nitrogenase and an alternative Mo-free iron-only nitrogenase, enabling this diazotroph to grow with molecular dinitrogen (N2) as the sole nitrogen source. Here, the Mo responses of the wild type and of a mutant lacking ModABC, the high-affinity molybdate transporter, were examined by proteome profiling, Western analysis, epitope tagging, and lacZ reporter fusions. Many Mo-controlled prot ...[more]

PMID: 26644433

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Project description:The current global situation points to the trend of the aging population. Along with the growth of population diseases, the pressure to develop new cell-based or drug-based therapies is increasing. One from main challenges in the field of regenerative medicine is large bones healing and osteoporosis, mainly postmenopausal women, treatment. To understand molecular processes which underlying bone development and turnover, osteogenic differentiation using specific media was applied. Differentiation process imitating real osteogenesis is associated with proteome and metabolome changes resulting in various signaling pathways activation. Proteome was profiled by ultrahigh-performance liquid chromatography and comprehensively quantified by ion mobility enhanced mass spectrometry. From 2267 reproducibly quantified proteins, 432 were differentially abundant by strict statistic and effect size criteria. Metabolome profiling was carried out nuclear magnetic resonance in resulting 27 reproducibly quantified metabolites. From them, 8 were statistically significant and differentially abundant. KEGG and MetaboAnalyst analysis indicated several metabolic pathways involved in osteogenic process. Enrichment analysis of differentialy abundant proteins reported PPAR, FoxO, IL-7 signaling pathways, thyroid hormones and steroid biosynthesis, mineral absorption, JAK-STAT and fatty acid degradation pathways as cascades with prominent impact to osteoinduction. Among them, enrichment analysis of significant metabolites showed Aminoacyl-tRNA biosynthesis as well as Valine, leucine, isoleucine metabolism together with phenylalanine, tyrosine and tryptophane metabolism as pathways which promotes osteodifferentiation. Moreover, we also selected few identified pathways which do not reported enrichment score, but have been described in the literature as majority. These cascades underline the complexity of the whole potential osteogenic mechanism. We also proposed promising targets for next validation in patient samples, what is step forward to influence of bone and/or oral defects.

Dataset Information

Proteome profiling of Rhodobacter capsulatus wild type and a ΔmodABC mutant in response to molybdenum

Publications

Proteome Profiling of the Rhodobacter capsulatus Molybdenum Response Reveals a Role of IscN in Nitrogen Fixation by Fe-Nitrogenase.

Similar Datasets

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