Proteomics

Dataset Information

78

Proteome profiling of Rhodobacter capsulatus wild type and a ΔmodABC mutant in response to molybdenum


ABSTRACT: The diazotrophic bacterium Rhodobacter capsulatus is able to synthesize two nitrogenases, a molybdenum-dependent and an alternative Mo-free iron-only nitrogenase, enabling growth with molecular dinitrogen (N2) as sole nitrogen source. The Mo response of the wild type and a mutant lacking the high-affinity molybdate transporter, ModABC, were analyzed by proteome profiling.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Rhodobacter capsulatus SB 1003  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Disease Free

SUBMITTER: Sina Schäkermann  

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD003123 | Pride | 2015-12-14

REPOSITORIES: Pride

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Publications

Proteome Profiling of the Rhodobacter capsulatus Molybdenum Response Reveals a Role of IscN in Nitrogen Fixation by Fe-Nitrogenase.

Hoffmann Marie-Christine MC   Wagner Eva E   Langklotz Sina S   Pfänder Yvonne Y   Hött Sina S   Bandow Julia E JE   Masepohl Bernd B  

Journal of bacteriology 20151207 4


Rhodobacter capsulatus is capable of synthesizing two nitrogenases, a molybdenum-dependent nitrogenase and an alternative Mo-free iron-only nitrogenase, enabling this diazotroph to grow with molecular dinitrogen (N2) as the sole nitrogen source. Here, the Mo responses of the wild type and of a mutant lacking ModABC, the high-affinity molybdate transporter, were examined by proteome profiling, Western analysis, epitope tagging, and lacZ reporter fusions. Many Mo-controlled proteins identified in  ...[more]

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