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Lipases from Metagenomic Sources

ABSTRACT: Functional Metaproteomic approach was used to discover lipolytic enzymes.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Soil Metagenome

SUBMITTER: Premankur Sukul

LAB HEAD: Prof. Dr. Lars I. Leichert

PROVIDER: PXD005148 | Pride | 2017-03-09

REPOSITORIES: Pride

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Simple discovery of bacterial biocatalysts from environmental samples through functional metaproteomics.

Sukul Premankur P Schäkermann Sina S Bandow Julia E JE Kusnezowa Anna A Nowrousian Minou M Leichert Lars I LI

Microbiome 20170303 1

Bacterial biocatalysts play a key role in our transition to a bio-based, post-petroleum economy. However, the discovery of new biocatalysts is currently limited by our ability to analyze genomic information and our capacity of functionally screening for desired activities. Here, we present a simple workflow that combines functional metaproteomics and metagenomics, which facilitates the unmediated and direct discovery of biocatalysts in environmental samples. To identify the entirety of lipolytic ...[more]

PMID: 28253936

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