Proteomics,Multiomics

Dataset Information

0

Two unrelated 8-vinyl reductases ensure production of mature chlorophylls in Acaryochloris marina


ABSTRACT: The aim of this project is to identify, by bottom-up proteomic analysis, one or both representatives of two classes of 8-vinyl reductase in an Acaryochloris marina cell lysate. This enzyme is responsible for the conversion of the C8 vinyl group in the chlorophyll precursor Mg-divinyl-protochlorophyllide to ethyl as part of the chlorphyll biosynthesis pathway. Both putative 8VRs, NmrA and FrhB were detected.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Acaryochloris Marina (strain Mbic 11017)

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Philip Jackson  

LAB HEAD: Prof. C Neil Hunter, FRS

PROVIDER: PXD003139 | Pride | 2020-05-26

REPOSITORIES: Pride

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Publications

Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen Guangyu E GE   Hitchcock Andrew A   Jackson Philip J PJ   Chaudhuri Roy R RR   Dickman Mark J MJ   Hunter C Neil CN   Canniffe Daniel P DP  

Journal of bacteriology 20160414 9


<h4>Unlabelled</h4>The major photopigment of the cyanobacterium Acaryochloris marina is chlorophyll d, while its direct biosynthetic precursor, chlorophyll a, is also present in the cell. These pigments, along with the majority of chlorophylls utilized by oxygenic phototrophs, carry an ethyl group at the C-8 position of the molecule, having undergone reduction of a vinyl group during biosynthesis. Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known t  ...[more]

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