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RSK feedback modulates initiation of embryonic stem cell differentiation

ABSTRACT: In this study we use a SILAC based quantitative proteomics approach to examine the ERK-responsive phosphoproteome in mouse embryonic stem cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Isabelle Nett

LAB HEAD: Kathryn Lilley

PROVIDER: PXD003247 | Pride | 2018-06-18

REPOSITORIES: Pride

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Negative feedback via RSK modulates Erk-dependent progression from naïve pluripotency.

Nett Isabelle Re IR Mulas Carla C Gatto Laurent L Lilley Kathryn S KS Smith Austin A

EMBO reports 20180612 8

Mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signalling is implicated in initiation of embryonic stem (ES) cell differentiation. The pathway is subject to complex feedback regulation. Here, we examined the ERK-responsive phosphoproteome in ES cells and identified the negative regulator RSK1 as a prominent target. We used CRISPR/Cas9 to create combinatorial mutations in <i>RSK</i> family genes. Genotypes that included homozygous null mutations in <i>Rps6ka1, ...[more]

PMID: 29895711

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