Proteomics

Dataset Information

15

Human follicular fluid lc-ms/ms


ABSTRACT: This study aimed to reveal if peptide profiling of individual human follicular fluid could become a new non-invasive predictive biomarker for oocyte quality, and attempted to discover candidate biomarkers for fertilization. In biomarker studies, because of normal clinical or biological variabil-ity, candidate biomarkers need to be validated across a large number of samples. To ensure that the discovered biomarkers are truly associated with oocytes quality and fertilization rather than the result of variability, three independent experiments were designed with a large population. In the present study, we investigated the peptide profile of human follicular fluid with fertilized and non-fertilized oocytes from patients undergoing in vitro fertilization using proteomic analysis with liquid chromatography-tandem mass spectrometry. We additionally determined the protein identi-ties of the discovered peptide biomarkers as a first step toward understanding the pathways in which they may function.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo sapiens  

TISSUE(S): Follicular Fluid

DISEASE(S): Not Available

SUBMITTER: Fang Chen  

PROVIDER: PXD003340 | Pride | 2018-10-26

REPOSITORIES: Pride

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Publications

Follicular fluid biomarkers for human in vitro fertilization outcome: Proof of principle.

Chen Fang F   Spiessens Carl C   D'Hooghe Thomas T   Peeraer Karen K   Carpentier Sebastien S  

Proteome science 20161114


Human follicular fluid (FF) is a unique biological fluid in which the oocyte develops in vivo, and presents an optimal source for non-invasive biochemical predictors. Oocyte quality directly influences the embryo development and hence, may be used as a predictor of embryo quality. Peptide profiling of FF and its potential use as a biomarker for oocyte quality has never been reported.This study screened FF for peptide biomarkers that predict the outcome of in vitro fertilization (IVF). Potential  ...[more]

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