Quantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique

ABSTRACT: We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total of eight iTRAQ 8-plex experiments were performed.

INSTRUMENT(S):

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Culture

SUBMITTER: Guijie Zhu  

LAB HEAD: Norman Dovichi

PROVIDER: PXD003462 | Pride | 2016-07-20

REPOSITORIES: pride