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A proteomic approach to identify endosomal cargoes controlling cancer invasiveness

ABSTRACT: We have developed a proteomic approach to identify endosomal cargoes that contribute to cancer invasiveness.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Sara Zanivan

LAB HEAD: Sara Zanivan

PROVIDER: PXD003745 | Pride | 2017-01-18

REPOSITORIES: Pride

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A proteomic approach to identify endosomal cargoes controlling cancer invasiveness.

Diaz-Vera Jesica J Palmer Sarah S Hernandez-Fernaud Juan Ramon JR Dornier Emmanuel E Mitchell Louise E LE Macpherson Iain I Edwards Joanne J Zanivan Sara S Norman Jim C JC

Journal of cell science 20170106 4

We have previously shown that Rab17, a small GTPase associated with epithelial polarity, is specifically suppressed by ERK2 (also known as MAPK1) signalling to promote an invasive phenotype. However, the mechanisms through which Rab17 loss permits invasiveness, and the endosomal cargoes that are responsible for mediating this, are unknown. Using quantitative mass spectrometry-based proteomics, we have found that knockdown of Rab17 leads to a highly selective reduction in the cellular levels of a ...[more]

PMID: 28062852

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