Proteomics

Dataset Information

88

Black pepper proteome


ABSTRACT: In this study, we have evaluated the proteomic changes that occur in Piper nigrum L.(black pepper) after infection by the pathogen Phytophthora capsici. We report novel leaf proteins from black pepper identified by an integrated transcriptome-assisted label-free quantitative proteomics pipeline. Several previously described methods were used to create this data set. Detached leaves were inoculated with either mock treatment, or the oomycete pathogen and small tissue samples only around the site of inoculation were collected for protein sample preparations. In order to quantify protein abundance in the samples being compared, we used a label free method of spiking samples with a known ratio of pre-digested peptide samples to normalize endogenous protein abundance in the MS detection. Our study attempts to explain the basal immune components of black pepper when challenged with P. capsici.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Piper nigrum  

TISSUE(S): Leaf

DISEASE(S): Not Available

SUBMITTER: Chidambareswaren Mahadevan  

LAB HEAD: Manjula Sakuntala

PROVIDER: PXD003887 | Pride | 2016-07-07

REPOSITORIES: Pride

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Publications

Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum-Phytophthora capsici Phytopathosystem.

Mahadevan Chidambareswaren C   Krishnan Anu A   Saraswathy Gayathri G GG   Surendran Arun A   Jaleel Abdul A   Sakuntala Manjula M  

Frontiers in plant science 20160620


Black pepper (Piper nigrum L.), a tropical spice crop of global acclaim, is susceptible to Phytophthora capsici, an oomycete pathogen which causes the highly destructive foot rot disease. A systematic understanding of this phytopathosystem has not been possible owing to lack of genome or proteome information. In this study, we explain an integrated transcriptome-assisted label-free quantitative proteomics pipeline to study the basal immune components of black pepper when challenged with P. capsi  ...[more]

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