Dataset Information

Proteomes of Noumeavirus and Melbournevirus particles

ABSTRACT: Melbournevirus and Noumeavirus are Marseilleviridae, the first isolated in Australia and the second in New Caledonia. The analysis of their particle proteomes give novel insights on their structures and protein contents.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Unclassified Marseilleviridae

SUBMITTER: Yohann Couté

LAB HEAD: Bruley

PROVIDER: PXD003910 | Pride | 2017-02-09

REPOSITORIES: Pride

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Publications

Noumeavirus replication relies on a transient remote control of the host nucleus.

Fabre Elisabeth E Jeudy Sandra S Santini Sébastien S Legendre Matthieu M Trauchessec Mathieu M Couté Yohann Y Claverie Jean-Michel JM Abergel Chantal C

Nature communications 20170421

Acanthamoeba are infected by a remarkable diversity of large dsDNA viruses, the infectious cycles of which have been characterized using genomics, transcriptomics and electron microscopy. Given their gene content and the persistence of the host nucleus throughout their infectious cycle, the Marseilleviridae were initially assumed to fully replicate in the cytoplasm. Unexpectedly, we find that their virions do not incorporate the virus-encoded transcription machinery, making their replication nuc ...[more]

PMID: 28429720

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Project description:Atmospheric particulate matter (PM) is a recognized risk factor for the global burden of disease in human populations. We are presenting here the application of toxicogenomics in the evaluation of the toxic effects of organic content of atmospheric particle matter (PM), from urban and rural environments (city of Barcelona and village of La Pobla, NE Spain), using the developing zebrafish embryo. The main goal is to identify the metabolic pathways involved in the adverse effects observed in zebrafish embryos exposed to PM organic content from urban and rural environments, also allowing the selection of genes of interest that are differentially expressed. The relevance of particle size to the PM toxicity is also addressed. Indeed, the zebrafish embryos were exposed to PM of aerodynamic diameter larger than 7.2 μm and smaller than 0.5 μm (PM10 and PM0.5, respectively). PM0.5 concentrated biological and toxic activities linked to organic substances. Transcriptomic analyses showed strong induction of the AhR signalling pathway (a.k.a. dioxin-like activity) for embryos exposed to both rural and urban extracts, correlating with the concentrations of PAHs. Urban extracts, with strong contribution of traffic emissions, specifically de-regulated oxidative stress-related genes, as well as pancreatic and eye-lens specific genes, two organs known to be affected by air pollution in humans. Exposure to rural extracts, with high contribution of wood burning emissions, affected genes implicated in basic cellular functions, in agreement with their strong embryotoxicity. Extracts from rural and urban samples elicited both common and specific transcriptome responses, suggesting different potentially adverse outcomes depending on PM source and composition. The authors thank the financial support of the Spanish Ministry (project TEA-PARTICLE, grant number CGL2011-29621) and the Portuguese Foundation for Science and Technology for the doctoral grant of Sofia R. Mesquita (SFRH/BD/80710/2011) funded by the Program POPH-QREN through the Portuguese Ministry of Education and Science and the European Social Fund, and support through project PEst-C/MAR/LA0015/2013.

Dataset Information

Proteomes of Noumeavirus and Melbournevirus particles

Publications

Noumeavirus replication relies on a transient remote control of the host nucleus.

Similar Datasets

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