Proteomics

Dataset Information

73

Proteomic analysis of distinct regions of human carotid atherosclerotic plaques


ABSTRACT: Protein profiles were determined from different distinct regions of human atherosclerotic plaques, including: internal control, fatty streak, plaque shoulder, plaque centre, and fibrous cap. Protein extracts from all 5 regions, from 3 men and 3 women, were separated by two-dimensional gel electrophoresis (in total 30 2-DE gels). Stained gels were matched by protein spot distribution, and a protein mapping was performed to identify as many protein spots as possible, by MALDI-TOF MS / peptide mass fingerprinting (Voyager DE Pro; Applied Biosystems). This resulted in the identification of 97 protein spots, corresponding to 52 unique protein by accession number. To expand on this nLC-MS/MS (Easy-nLC / LTQ Orbitrap Velos Pro; Thermo Fisher Scientific) experiments were run on additional carotid plaque samples from 10 men and 10 women, using three of the predefined regions; internal control, fatty streak, plaque core. MS/MS analysis resulted in the identification of over 1000 proteins. Human carotid atherosclerotic plaques (n = 26) were obtained from the Linköping Carotid Study, and all methods/protocols described herein have been approved by the local ethics committee (Linköping University Hospital, Linköping, Sweden). Written informed consent was also obtained from all patients.

INSTRUMENT(S): LTQ Orbitrap Velos, Voyager-DE PRO

ORGANISM(S): Homo sapiens  

TISSUE(S): Carotid Atherosclerotic Plaque

DISEASE(S): Atherosclerosis

SUBMITTER: Liam Ward  

LAB HEAD: Xi-Ming Yuan

PROVIDER: PXD003930 | Pride | 2016-05-24

REPOSITORIES: Pride

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Publications

Distinctive proteomic profiles among different regions of human carotid plaques in men and women.

Liang Wenzhao W   Ward Liam J LJ   Karlsson Helen H   Ljunggren Stefan A SA   Li Wei W   Lindahl Mats M   Yuan Xi-Ming XM  

Scientific reports 20160520


The heterogeneity of atherosclerotic tissue has limited comprehension in proteomic and metabolomic analyses. To elucidate the functional implications, and differences between genders, of atherosclerotic lesion formation we investigated protein profiles from different regions of human carotid atherosclerotic arteries; internal control, fatty streak, plaque shoulder, plaque centre, and fibrous cap. Proteomic analysis was performed using 2-DE with MALDI-TOF, with validation using nLC-MS/MS. Protein  ...[more]

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