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Dynamic of the Grb2 interactome in primary cells by DDA-MS

ABSTRACT: We applied AP-MS to quantify Grb2 interaction dynamics in primary T cells. Data generated here in shotgun/DDA mode were used to build a high quality Grb2 binders-specific SWATH assay library for high-throughput targeted analysis of DIA data.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, T Cell, Lymph Node

SUBMITTER: Etienne Caron

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD003973 | Pride | 2017-03-09

REPOSITORIES: Pride

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Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry.

Caron Etienne E Roncagalli Romain R Hase Takeshi T Wolski Witold E WE Choi Meena M Menoita Marisa G MG Durand Stephane S García-Blesa Antonio A Fierro-Monti Ivo I Sajic Tatjana T Heusel Moritz M Weiss Tobias T Malissen Marie M Schlapbach Ralph R Collins Ben C BC Ghosh Samik S Kitano Hiroaki H Aebersold Ruedi R Malissen Bernard B Gstaiger Matthias M

Cell reports 20170301 13

Spatiotemporal organization of protein interactions in cell signaling is a fundamental process that drives cellular functions. Given differential protein expression across tissues and developmental stages, the architecture and dynamics of signaling interaction proteomes is, likely, highly context dependent. However, current interaction information has been almost exclusively obtained from transformed cells. In this study, we applied an advanced and robust workflow combining mouse genetics and af ...[more]

PMID: 28355572

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