Proteomics

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Mesenchymal Stromal Cells: What are the secrets of their secretome?

ABSTRACT: Multipotent Mesenchymal Stromal Cells (MSCs) are multipotent adult cells that can be isolated from different tissues. The current hypothesis supports that the main MSCs action mechanism relates to its paracrine activity, creating a microenvironment with trophic signals. However, preclinical studies with different sources of MSCs and different animal models have shown conflicting results. Therefore, the evaluation of these cells secretome content is of great interest. Here we analyzed the secreted proteins of MSCs, isolated from 3 different sources (adipose tissue, uterine tubes and skeletal muscle), obtained from 5 different donors. Following MSCs characterization, proteins secreted in serum-free conditions (conditioned media, CM) were analyzed by mass-spectrometry-based quantitative proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Whole Body, Mesenchymal Cell

SUBMITTER: Amanda Assoni  

LAB HEAD: Mayana Zatz

PROVIDER: PXD003978 | Pride | 2016-12-09

REPOSITORIES: Pride

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Publications

Different Donors Mesenchymal Stromal Cells Secretomes Reveal Heterogeneous Profile of Relevance for Therapeutic Use.

Assoni Amanda A   Coatti Giuliana G   Valadares Marcos C MC   Beccari Melinda M   Gomes Juliana J   Pelatti Mayra M   Mitne-Neto Miguel M   Carvalho Valdemir M VM   Zatz Mayana M  

Stem cells and development 20161020 3

Duchenne muscular dystrophy (DMD) is a lethal X-linked disorder caused by null mutations in the dystrophin gene. Although the primary defect is the deficiency of muscle dystrophin, secondary events, including chronic inflammation, fibrosis, and muscle regeneration failure are thought to actively contribute to disease progression. Despite several advances, there is still no effective therapy for DMD. Therefore, the potential regenerative capacities, and immune-privileged properties of mesenchymal  ...[more]

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