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Metaproteomic Monitoring of Perinatal Mouse Gut Microbiota

ABSTRACT: In the presented study, in order to unravel gut microbial community multiplicity and the influence of maternal milk nutrients (i.e., IgA) on gut mucosal microbiota onset and shaping, a mouse GM (MGM) was used as newborn study model to discuss genetic background and feeding modulation on gut microbiota in term of symbiosis, dysbiosis and rebiosis maintenance during early gut microbiota onset and programming after birth. Particularly, a bottom-up shotgun metaproteomic approach, combined with a computational pipeline, has been compred with a culturomics analysis of mouse gut microbiota, obtained by MALDI-TOF mass spectrometry (MS).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bacteria

TISSUE(S): Gut

SUBMITTER: Stefano Levi Mortera

LAB HEAD: Lorenza Putignani

PROVIDER: PXD004033 | Pride | 2016-10-13

REPOSITORIES: Pride

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Monitoring Perinatal Gut Microbiota in Mouse Models by Mass Spectrometry Approaches: Parental Genetic Background and Breastfeeding Effects.

Levi Mortera Stefano S Del Chierico Federica F Vernocchi Pamela P Rosado Maria M MM Cavola Agnese A Chierici Marco M Pieroni Luisa L Urbani Andrea A Carsetti Rita R Lante Isabella I Dallapiccola Bruno B Putignani Lorenza L

Frontiers in microbiology 20160926

At birth, contact with external stimuli, such as nutrients derived from food, is necessary to modulate the symbiotic balance between commensal and pathogenic bacteria, protect against bacterial dysbiosis, and initiate the development of the mucosal immune response. Among a variety of different feeding patterns, breastfeeding represents the best modality. In fact, the capacity of breast milk to modulate the composition of infants' gut microbiota leads to beneficial effects on their health. In thi ...[more]

PMID: 27725814

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