Dataset Information

Multiplexed temporal quantification of the human plasma peptidome

ABSTRACT: The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and post-translational modifications and is a rich source of biomarkers and novel bioactives. To examine the utility of peptidomics we have examined the effects of exercise on the peptidome because exercise is thought to convey many of its physiological benefits via the blood stream. Deep peptidomic analysis was achieved using 2D LC-MS/MS with multiple fragmentation methods and included identification of post-translational modifications and multiplexed temporal quantification. We show the plasma peptidome is subject to transient changes during exercise on a time-scale of minutes that is rapidly reversible during recovery. Known peptide hormones were acutely regulated including insulin, glucagon, ghrelin, bradykinin, cholecystokinin, secretogranins and many others. Acute regulation of proteases and post-translational modifications including glycosylation were observed. These data reveal the power of peptidomic quantification to temporally quantify bioactive peptides, biomarkers and proteolytic activity during pathophysiological processes.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Benjamin Parker

LAB HEAD: Benjamin Parker

PROVIDER: PXD004781 | Pride | 2017-11-21

REPOSITORIES: Pride

ACCESS DATA

Publications

Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome.

Parker Benjamin L BL Burchfield James G JG Clayton Daniel D Geddes Thomas A TA Payne Richard J RJ Kiens Bente B Wojtaszewski Jørgen F P JFP Richter Erik A EA James David E DE

Molecular & cellular proteomics : MCP 20171005 12

Exercise is extremely beneficial to whole body health reducing the risk of a number of chronic human diseases. Some of these physiological benefits appear to be mediated via the secretion of peptide/protein hormones into the blood stream. The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and PTMs, and is a rich source of hormones. In the current study we have quantified the effects of intense exercise on the ...[more]

PMID: 28982716

Similar Datasets

Project description:The rates of obesity and sedentary lifestyle are on a dramatic incline, with associated detrimental health effects among women in particular. Although exercise prescriptions are useful for overcoming these problems, success can be hampered by differential responsiveness among individuals in cardiovascular fitness indices (i.e., improvements in strength, lipids, VO2max). Genetic factors appear to play an important role in determining this inter-individual variation in responsiveness. We performed microarray analyses on mRNA in whole blood from 60 sedentary women from a multi-ethnic cohort who underwent 12 weeks of exercise, to identify gene subsets that were differentially expressed between individuals who experienced the greatest and least improvements in fitness based upon a composite fitness score index. We identified 43 transcripts in 39 unique genes (FDR<10%; FC>1.5) whose expression increased the most in “high” versus “low” premenopausal female responders. Several (TIGD7, UQCRH, PSMA6, WDR12, TFB2M, USP15) have reported associations with fitness-related phenotypes. Bioinformatic analysis of the 39 genes identified 4 miRNAs whose expression has been linked to cardiovascular diseases (ANKRD22: miR-637, LRRFIP1: miR-132, PRKAR2B: miR-92a, RSAD2:miR-192). These 39 genes were enriched in 6 biological pathways, including the oxidative phosphorylation pathway (p=8.08 x 10-3). Two genes, LRRFIP1 and SNORD30, were also identified with lower expression in high responding postmenopausal women. In summary, we identified gene signatures based on mRNA analysis that define responsiveness to exercise in a largely minority-based female cohort. Importantly, this study validates several genes/pathways previously associated with exercise responsiveness and extends these findings with additional novel genes. We performed microarray analyses on mRNA in whole blood from 60 sedentary women from a multi-ethnic cohort who underwent 12 weeks of exercise, to identify gene subsets that were differentially expressed between individuals who experienced the greatest and least improvements in fitness based upon a composite fitness score index.

Dataset Information

Multiplexed temporal quantification of the human plasma peptidome

Publications

Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure