Proteomics

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Enhanced limonene production in Synechococcus elongatus PCC 7942

ABSTRACT: In this study, through computational modeling and synthetic biology tools, we identified and overcame a key flux controlling node in MEP-derived terpene biosynthesis in cyanobacteria. This strategy bypasses the limitation in traditional stepwise metabolic engineering, and led to significantly higher terpene productivity. Moreover, our results revealed photosynthesis limitations during high level of terpene production. We suggest that fine-tuning energy and reductant requirement could be a key factor in establishing a viable ‘non-native’ terpene sink in photosynthetic systems.

INSTRUMENT(S): LTQ

ORGANISM(S): Synechococcus Elongatus (strain Pcc 7942) (anacystis Nidulans R2)

SUBMITTER: XIN WANG  

LAB HEAD: Joshua S. Yuan & Susie Y. Dai

PROVIDER: PXD005105 | Pride | 2016-12-07

REPOSITORIES: Pride

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Enhanced limonene production in cyanobacteria reveals photosynthesis limitations.

Wang Xin X   Liu Wei W   Xin Changpeng C   Zheng Yi Y   Cheng Yanbing Y   Sun Su S   Li Runze R   Li Runze R   Zhu Xin-Guang XG   Dai Susie Y SY   Rentzepis Peter M PM   Yuan Joshua S JS  

Proceedings of the National Academy of Sciences of the United States of America 20161123 50

Terpenes are the major secondary metabolites produced by plants, and have diverse industrial applications as pharmaceuticals, fragrance, solvents, and biofuels. Cyanobacteria are equipped with efficient carbon fixation mechanism, and are ideal cell factories to produce various fuel and chemical products. Past efforts to produce terpenes in photosynthetic organisms have gained only limited success. Here we engineered the cyanobacterium Synechococcus elongatus PCC 7942 to efficiently produce limon  ...[more]

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