AP-MS in CRISPR/Cas9 Utilizing Systems for Mapping Endogenous Protein Complexes

ABSTRACT: We combined clustered regularly interspaced short palindromic repeats/Cas9-mediated gene knockout technology with affinity purification using antibodies against endogenous proteins followed by mass spectrometry analysis, to sensitively and precisely detect protein-protein interactions in unaltered in vivo settings. Using this system, we analyzed endogenous neurofibromin-associated protein complexes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture, Hela Cell

DISEASE(S): Cervix Carcinoma,Disease Free

SUBMITTER: Xu Li  

LAB HEAD: Junjie Chen

PROVIDER: PXD005107 | Pride | 2017-03-15

REPOSITORIES: Pride