Proteomics

Dataset Information

SMAD2/3 interactome in human pluripotent stem cells

ABSTRACT: This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from nuclear extracts in these two conditions using a specific antibody. Non-immune IgG immunoprecipitations were performed as negative controls. Three biological replicates per conditions were analyzed by quantitative label-free mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Multipotent Stem Cell, Stem Cell, Cell Culture, Embryonic Stem Cell

SUBMITTER: Alessandro Bertero  

LAB HEAD: Ludovic Vallier

PROVIDER: PXD005285 | Pride | 2018-01-04

REPOSITORIES: Pride

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20130626_Q1_SA_NiHu_Mendjan_pluripotent_IgG_1.raw Raw
20130626_Q1_SA_NiHu_Mendjan_pluripotent_IgG_2.raw Raw
20130626_Q1_SA_NiHu_Mendjan_pluripotent_IgG_3.raw Raw
20130626_Q1_SA_NiHu_Mendjan_pluripotent_Smad2_1.raw Raw
20130626_Q1_SA_NiHu_Mendjan_pluripotent_Smad2_2.raw Raw
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