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SMAD2/3 interactome in human pluripotent stem cells

ABSTRACT: This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from nuclear extracts in these two conditions using a specific antibody. Non-immune IgG immunoprecipitations were performed as negative controls. Three biological replicates per conditions were analyzed by quantitative label-free mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Multipotent Stem Cell, Stem Cell, Cell Culture, Embryonic Stem Cell

SUBMITTER: Alessandro Bertero

LAB HEAD: Ludovic Vallier

PROVIDER: PXD005285 | Pride | 2018-01-04

REPOSITORIES: Pride

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The SMAD2/3 interactome reveals that TGFβ controls m<sup>6</sup>A mRNA methylation in pluripotency.

Bertero Alessandro A Brown Stephanie S Madrigal Pedro P Osnato Anna A Ortmann Daniel D Yiangou Loukia L Kadiwala Juned J Hubner Nina C NC de Los Mozos Igor Ruiz IR Sadée Christoph C Lenaerts An-Sofie AS Nakanoh Shota S Grandy Rodrigo R Farnell Edward E Ule Jernej J Stunnenberg Hendrik G HG Mendjan Sasha S Vallier Ludovic L

Nature 20180228 7695

The TGFβ pathway has essential roles in embryonic development, organ homeostasis, tissue repair and disease. These diverse effects are mediated through the intracellular effectors SMAD2 and SMAD3 (hereafter SMAD2/3), whose canonical function is to control the activity of target genes by interacting with transcriptional regulators. Therefore, a complete description of the factors that interact with SMAD2/3 in a given cell type would have broad implications for many areas of cell biology. Here we ...[more]

PMID: 29489750

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