Dataset Information

Tripartite symbiosis formed by Pisum sativum, rhizobia and mycorrhiza: Implications for the symbionts, the host plant and the pathogenic fungi, part 2

ABSTRACT: The lower needs for chemical fertilisers make the Pisum Sativum well suited for sustainable agricultural systems. During the past decades, yield losses were mainly due to pathogenic infestation, with Ascochyta blight as the most severe disease. In this fungal disease complex Didymella pinodes, as the most aggressive pathogen, has been examined intensively. Despite the great number of varieties, no resistance was yet found and several strategies were developed to avoid infestation. In recent years enhanced resistance to pathogens due to previous infestation by symbiotic microorganisms was discovered in several plants. However, only little attention was drawn on the implications of P. Sativum´s symbionts (Rhizobia, Mycorrhiza) on the disease severity as well as the effects of the pathogen on the symbiotic interactions. Hence, here the implications of the pathogenic infestation on the tripartite symbiosis of P. Sativum on a molecular (proteomics, metabolomics) and physiological level, and moreover, the potential of the root symbionts to enhance resistance against Didymella pinodes are investigated.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Pisum Sativum (garden Pea)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Reinhard Turetschek

LAB HEAD: Stefanie Wienkoop

PROVIDER: PXD005701 | Pride | 2017-01-16

REPOSITORIES: Pride

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Publications

Microbial symbionts affect Pisum sativum proteome and metabolome under Didymella pinodes infection.

Desalegn G G Turetschek R R Kaul H-P HP Wienkoop S S

Journal of proteomics 20160322

<h4>Unlabelled</h4>The long cultivation of field pea led to an enormous diversity which, however, seems to hold just little resistance against the ascochyta blight disease complex. The potential of below ground microbial symbiosis to prime the immune system of Pisum for an upcoming pathogen attack has hitherto received little attention. This study investigates the effect of beneficial microbes on the leaf proteome and metabolome as well as phenotype characteristics of plants in various symbiont ...[more]

PMID: 27016040

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Project description:Bathymodiolus mussels inhabiting deep-sea hydrothermal vents harbor bacterial symbionts in their gills, which support the animals’ diet. While the basic mechanisms of energy generation and CO2 fixation that drive these symbioses are largely established, details of molecular interactions between the symbiotic partners and adaptations to their respective habitats remain unknown. In this study, we therefore comparatively examined the genomes and proteomes of two Bathymodiolus hosts and their respective symbionts from different geographical locations. Two mussel species were proteomically compared: i) B. thermophilus mussel containing sulfur-oxidizing symbiont from the east pacific rise. thermophilus and ii) B. azoricus containing thiotrophic and methanotrophic symbionts from the mid-atlantic ridge. Symbionts (for both species) and host components (for B. azoricus) were selectively enriched using a multi-step centrifugation procedure. Enriched host and symbiont fractions along with unenriched gill foot tissue were subject to in-depth semi-quantitative proteomic analyses using the orbitrap and velos mass spectrometers. Proteins were quantified based on their spectral counts using the normalized spectral abundance factor (NSAF) method. We identified common strategies of metabolic interactions that provide mutual nutritional support between host and symbionts, such as the detoxification of ambient sulfide by the Bathymodiolus host, which provides a stable thiosulfate reservoir for the thiotrophic symbionts, and a putative amino acid cycling mechanism that could supply the host with symbiont-derived amino acids. A suite of genes and proteins putatively related to virulence or defense functions was particularly abundant in the B. thermophilus symbiont, compared to its symbiont relatives, and may pose a host species-specific adaptation. Our results reveal both, a high degree of integration between the symbiotic partners, and great potential to adapt to the prevailing environment, which facilitate the holobiont’s survival in its hydrothermal vent habitat.

Dataset Information

Tripartite symbiosis formed by Pisum sativum, rhizobia and mycorrhiza: Implications for the symbionts, the host plant and the pathogenic fungi, part 2

Publications

Microbial symbionts affect Pisum sativum proteome and metabolome under Didymella pinodes infection.

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