Characterisation of NBEAL2 interactome in HEK293T cells

ABSTRACT: Variants in NBEAL2 are causal of Grey Platelet Syndrome (GPS), a rare bleeding disorder characterized by absence of alpha- and specific- granules in platelets and neutrophils, respectively. The role of the scaffolding multidomain NBEAL2 protein in cell biology and granule homeostasis is unknown. We have performed proteomics to identify NBEAL2’s binding partners followed by different layers of validation including biochemical, cellular and functional analysis in vitro and in vivo.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Cell Culture, Embryonic Stem Cell

SUBMITTER: James Wright  

LAB HEAD: Jyoti Choudhary

PROVIDER: PXD006091 | Pride | 2018-02-14

REPOSITORIES: Pride