Proteomics

Dataset Information

60

Chemical probes unravel an antimicrobial defense response triggered by binding of the human opioid dynorphin to a bacterial sensor kinase


ABSTRACT: Host-microbe communication via small molecule signals is often poorly understood at the molecular level. Under conditions of host stress, levels of the human opioid peptide dynorphin are elevated, triggering virulence in the opportunistic pathogenic bacterium Pseudomonas aeruginosa (PAO1) via an unknown pathway. Here we apply multiple chemical biology strategies to unravel the mode of action of this putative interkingdom signal. We designed and applied dynorphin-inspired photoaffinity probes (DYN4 and DYN5) to reveal the protein targets of the peptide in live bacteria via chemical proteomics. Sensor kinase ParS was identified as a potential hit. Subsequent full proteome studies revealed that dynorphin(1-13) (DYN) induces an antimicrobial peptide-like response in Pseudomonas, with specific upregulation of membrane defence mechanisms (datasets Stat.10 and Exp.5). No such response was observed in a parS mutant (Stat.1), functionally linking DYN engagement with ParS to this phenotype.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Megan Wright  

LAB HEAD: Stephan A. Sieber

PROVIDER: PXD006172 | Pride | 2017-04-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20160128_MHW_B145_DMSO_A.raw Raw
20160128_MHW_B145_DMSO_B.raw Raw
20160128_MHW_B145_DMSO_C.raw Raw
20160128_MHW_B145_DYN4_A.raw Raw
20160128_MHW_B145_DYN4_B.raw Raw
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Publications

Chemical Probes Unravel an Antimicrobial Defense Response Triggered by Binding of the Human Opioid Dynorphin to a Bacterial Sensor Kinase.

Wright Megan H MH   Fetzer Christian C   Sieber Stephan A SA  

Journal of the American Chemical Society 20170420 17


Host-microbe communication via small molecule signals is important for both symbiotic and pathogenic relationships, but is often poorly understood at the molecular level. Under conditions of host stress, levels of the human opioid peptide dynorphin are elevated, triggering virulence in the opportunistic pathogenic bacterium Pseudomonas aeruginosa via an unknown pathway. Here we apply a multilayered chemical biology strategy to unravel the mode of action of this putative interkingdom signal. We d  ...[more]

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