S. cerevisiae proteome response to heat stress - 240 min time course. C4PR_LIV
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ABSTRACT: In this project, we follow the temporal proteome dynamics during heat stress response in a model organism Saccharomyces cerevisiae. Using label-free quantification we determine changes in the yeast proteome at different times after mild temperature shift, from 30˚C to 37˚C. The time points measured were 0 min (30˚C, no stress) and then at 10 min, 30 min, 60 min, 120 min and 240 min after cells were transferred to 37 °C. For each time point, four biological replicates were collected for the haploid wild type (BY4742, Mat ALPHA, his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; YJL088w::kanMX4) strain and a haploid strain with a chaperone Ssb1p deletion (ssb1Δ).
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Haploid Cell
SUBMITTER: Andrew Jarnuczak
LAB HEAD: Professor Simon Hubbard
PROVIDER: PXD006262 | Pride | 2018-01-17
REPOSITORIES: Pride
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