Dataset Information

Multiple Extracellular Vesicle Types in Peritoneal Dialysis Effluent are Prominent and Contain Known Biomarkers

ABSTRACT: Proteomics-based analysis of Peritoneal Dialysis Effluent is a relatively new approach to studying the changes associated with PD. Recent studies have shown altered proteomic profiles in various conditions of PD patients, including uremia, diabetes, peritonitis, abnormal peritoneal transport function and with different peritoneal dialysis modality. However, these studies extracted proteins in PDE by precipitation techniques resulting in high abundant diffusible proteins from blood circulation which interfered with the identification of low abundant, locally secreted, yet important, biomarkers. we hypothesize that EVs are secreted from peritoneal resident cells and contain known biomarkers for PDE-related membrane injury. As a proof of concept, here we identified and characterized EVs from PDE of healthy PD patients using transmission electron microscopy (TEM), dynamic light scattering (DLS), and nanoparticle tracking analysis (NTA). Furthermore, we did proteomics and bioinformatics analyses to determine the number and type of proteins measurable within EVs isolated from the PDE using two methods of vesicle isolation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Poorichaya Somparn

LAB HEAD: Trairak Pisitkun

PROVIDER: PXD006371 | Pride | 2018-08-14

REPOSITORIES: Pride

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Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers.

Pearson Lachlan James LJ Klaharn I-Yanut IY Thongsawang Bussakorn B Manuprasert Wasin W Saejew Thunvarat T Somparn Poorichaya P Chuengsaman Piyatida P Kanjanabuch Talerngsak T Pisitkun Trairak T

PloS one 20170608 6

Peritoneal dialysis inevitability results in activation of inflammatory processes and its efficiency is highly variable between patients. An improved method to isolate biomarkers and study pathophysiological mechanisms in peritoneal dialysis effluent (PDE) is expected to be of much benefit for the development of this treatment approach and help with patient management. Extracellular vesicles (EVs) are released as part of normal cellular processes. Their proteome is expected to reflect both type ...[more]

PMID: 28594924

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