Proteomics

Dataset Information

36

Subregion-specific proteomic signature in the hippocampus for memory formation in adult mice


ABSTRACT: The hippocampal formation is a brain structure essential for higher-order cognitive functions. It has exquisite differences in anatomical organization and cellular composition, and hippocampal sub-regions have different properties and functional roles. Areas CA1 and CA3 in particular, are key sub-regions for learning and memory formation that fulfill complementary but specific functions. The molecular basis for such specific properties and the link to learning and memory remain unknown. Here using a SWATH-MS proteomic approach and bioinformatic tools, we identify a selective proteomic signature in area CA1 and CA3, and reveal their specific dynamics during memory formation. We show that 30% of all quantifiable proteins are differentially expressed in area CA1 and CA3 at baseline, and that each proteome responds differently during the formation of memory for object or object location. Using clustering and cross-correlational analyses, we outline specific temporal proteomic profiles and an increased correlation between both forms of memory within area CA1, but not within area CA3. These results provide new insight into a proteomic basis for hippocampal sub-region molecular and functional specificity.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus musculus  

TISSUE(S): Brain

DISEASE(S): Not Available

SUBMITTER: Lukas von Ziegler  

LAB HEAD: Prof. Isabelle M. Mansuy

PROVIDER: PXD006382 | Pride | 2018-03-28

REPOSITORIES: Pride

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Publications

Subregion-Specific Proteomic Signature in the Hippocampus for Recognition Processes in Adult Mice.

von Ziegler Lukas M LM   Selevsek Nathalie N   Tweedie-Cullen Ry Y RY   Kremer Eloïse E   Mansuy Isabelle M IM  

Cell reports 20180301 12


The hippocampal formation is a brain structure essential for higher-order cognitive functions. It has a complex anatomical organization and cellular composition, and hippocampal subregions have different properties and functional roles. In this study, we used SWATH-MS to determine whether the proteomes of hippocampus areas CA1 and CA3 can explain the commonalities or specificities of these subregions in basal conditions and after recognition memory. We show that the proteomes of areas CA1 and CA  ...[more]

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