Dataset Information

Peptidomics analysis of saliva of the two-spotted spider mite Tetranychus urticae

ABSTRACT: Recently, we elucidated T. urticae’s repertoire of secreted salivary proteins, revealing several members of expanded protein families with unknown functions [PMID: 27703040]. In this study, mite salivary secretions were additionally examined using a peptidomics approach.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Tetranychus Urticae (two-spotted Spider Mite)

TISSUE(S): Saliva

SUBMITTER: Wannes Dermauw

LAB HEAD: Wannes Dermauw

PROVIDER: PXD006385 | Pride | 2017-09-14

REPOSITORIES: Pride

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Publications

A Gene Family Coding for Salivary Proteins (SHOT) of the Polyphagous Spider Mite Tetranychus urticae Exhibits Fast Host-Dependent Transcriptional Plasticity.

Jonckheere Wim W Dermauw Wannes W Khalighi Mousaalreza M Pavlidi Nena N Reubens Wim W Baggerman Geert G Tirry Luc L Menschaert Gerben G Kant Merijn R MR Vanholme Bartel B Van Leeuwen Thomas T

Molecular plant-microbe interactions : MPMI 20171102 1

The salivary protein repertoire released by the herbivorous pest Tetranychus urticae is assumed to hold keys to its success on diverse crops. We report on a spider mite-specific protein family that is expanded in T. urticae. The encoding genes have an expression pattern restricted to the anterior podocephalic glands, while peptide fragments were found in the T. urticae secretome, supporting the salivary nature of these proteins. As peptide fragments were identified in a host-dependent manner, we ...[more]

PMID: 29094648

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Project description:While pathogen-induced immunity is comparatively well characterized, far less is known about plant defense responses to arthropod herbivores. To date, most molecular-genetic studies of plant-arthropod interactions have focused on insects. However, plant-feeding (phytophagous) mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g., Lepidopteran larvae or aphids). The two-spotted spider mite, Tetranychus urticae, is among the most significant mite pests in agriculture. T. urticae is an extreme generalist that has been documented on a staggering number of plant hosts (more than 1,100), and is renowned for the rapid evolution of pesticide resistance. To understand reciprocal interactions between T. urticae and a plant host at the molecular level, we examined mite herbivory using Arabidopsis thaliana. Despite differences in feeding guilds, we found that transcriptional responses of A. thaliana to mite herbivory generally resembled those observed for insect herbivores. In particular, defense to mites was mediated by jasmonic acid (JA) biosynthesis and signaling. Further, indole glucosinolates dramatically increased mite mortality and development times. Variation in both basal and activated levels of these defense pathways might also explain differences in mite damage and feeding success between A. thaliana accessions. On the herbivore side, a diverse set of genes associated with detoxification of xenobiotics was induced upon exposure to increasing levels of in planta indole glucosinolates. Our findings provide molecular insights into the nature of, and response to, herbivory for a representative of a major class of arthropod herbivores. We used microarray to assess global gene expresion in Arabidopsis thaliana upon Tetranychus urticae attack in two A. thaliana accessions: Bla-2, resistant to spider mite herbivory and Kon, susceptible to spider mite herbivory. 3 week old Arabidopsis thaliana plants were subjected to Tetranychus urticae attack through application of 10 mites for various periods of time (timecourse scenario) or hundreds of mites for 1 hour (feeding site scenario).

Dataset Information

Peptidomics analysis of saliva of the two-spotted spider mite Tetranychus urticae

Publications

A Gene Family Coding for Salivary Proteins (SHOT) of the Polyphagous Spider Mite Tetranychus urticae Exhibits Fast Host-Dependent Transcriptional Plasticity.

Similar Datasets

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