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Shotgun mass-spectrometry of HL-60 cell proteins for spectrum library development

ABSTRACT: ATRA-induced differentiation of HL-60 cells was studied using targeted mass-spectrometry including selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) approach. PRM experiment was performed in time-course manner, without peptide standards usage. PRM data was inspected in Skyline 3.1 software. In order to check peptide identity we developed spectrum library based on shotgun mass-spectrometry data, which has been obtained for HL-60 cells protein samples at 0, 3, 24, 48 and 96h after ATRA treatment.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Svetlana Novikova

LAB HEAD: Victor Zgoda

PROVIDER: PXD006421 | Pride | 2017-11-09

REPOSITORIES: Pride

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Application of selected reaction monitoring and parallel reaction monitoring for investigation of HL-60 cell line differentiation.

Novikova Svetlana E SE Tikhonova Olga V OV Kurbatov Leonid K LK Farafonova Tatiana E TE Vakhrushev Igor V IV Zgoda Victor G VG

European journal of mass spectrometry (Chichester, England) 20170801 4

Targeted mass spectrometry represents a powerful tool for investigation of biological processes. The convenient approach of selected reaction monitoring using stable isotope-labeled peptide standard (SIS) is widely applied for protein quantification. Along with this method, high-resolution parallel reaction monitoring has been increasingly used for protein targeted analysis. Here we applied two targeted approaches (selected reaction monitoring with SIS and label-free parallel reaction monitoring ...[more]

PMID: 29028392

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